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Phagemid Vectors for Phage Display: Properties, Characteristics and Construction
Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2
2012-03-30
发表期刊JOURNAL OF MOLECULAR BIOLOGY
卷号417期号:3页码:129-143
摘要Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.; Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved.
文章类型Review
关键词Phage Display Phagemid Filamentous Phage Phagemid Vector Construction
学科领域生物传感器技术
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jmb.2012.01.038
关键词[WOS]COMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000301805800001
引用统计
被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1210
专题生物传感技术团队
作者单位1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
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Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143.
APA Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,Petrenko, Valery,&Liu, Aihua.(2012).Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.JOURNAL OF MOLECULAR BIOLOGY,417(3),129-143.
MLA Qi, Huan,et al."Phagemid Vectors for Phage Display: Properties, Characteristics and Construction".JOURNAL OF MOLECULAR BIOLOGY 417.3(2012):129-143.
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