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Title:
Phagemid Vectors for Phage Display: Properties, Characteristics and Construction
Author: Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2
Source: JOURNAL OF MOLECULAR BIOLOGY
Issued Date: 2012-03-30
Volume: 417, Issue:3, Pages:129-143
Keyword: phage display ; phagemid ; filamentous phage ; phagemid vector construction
Subject: 生物资源::生物传感器技术
DOI: 10.1016/j.jmb.2012.01.038
DOC Type: Review
Abstract: Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.
English Abstract: Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biochemistry & Molecular Biology
WOS Subject Extended: Biochemistry & Molecular Biology
WOS Keyword Plus: COMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION
Indexed Type: SCI
Language: 英语
WOS ID: WOS:000301805800001
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/1210
Appears in Collections:生物传感技术团队_期刊论文

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description.institution: 1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

Recommended Citation:
Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143.
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