A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942

doi:10.1128/AEM.01222-18

QIBEBT-IR

	A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942
	Lou, Wenjing 1; Tan, Xiaoming 1; Song, Kuo 1,3; Zhang, Shanshan 1; Luan, Guodong 1; Li, Cheng 1; Lu, Xuefeng 1,2
	2018-09-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	84 期号:18 页码:16
摘要	In response to a broad range of habitats and environmental stresses, cyanobacteria have evolved various effective acclimation strategies, which will be helpful for improving the stress tolerances of photosynthetic organisms, including higher plants. Synechococcus elongatus UTEX 2973 and PCC 7942 possess genomes that are 99.8% identical but exhibit significant differences in cell growth and stress tolerance. In this study, we found that a single amino acid substitution at FoF1 ATP synthase subunit alpha (AtpA), C252Y, is the primary contributor to the improved stress tolerance of S. elongatus UTEX 2973. Site-saturation mutagenesis experiments showed that point mutations of cysteine 252 to any of the four conjugated amino acids could significantly improve the stress tolerance of S. elongatus PCC 7942. We further confirmed that the C252Y mutation increases AtpA protein levels, intracellular ATP synthase activity, intracellular ATP abundance, transcription of psbA genes (especially psbA2), photosystem II activity, and glycogen accumulation in S. elongatus PCC 7942. This work highlights the importance of AtpA in improving the stress tolerance of cyanobacteria and provides insight into how cyanobacteria evolve via point mutations in the face of environmental selection pressures. IMPORTANCE Two closely related Synechococcus strains showed significantly different tolerances to high light and high temperature but limited genomic differences, providing us opportunities to identify key genes responsible for stress acclimation by a gene complementation approach. In this study, we confirmed that a single point mutation in the alpha subunit of FoF1 ATP synthase (AtpA) contributes mainly to the improved stress tolerance of Synechococcus elongatus UTEX 2973. The point mutation of AtpA, the important ATP-generating complex of photosynthesis, increases AtpA protein levels, intracellular ATP synthase activity, and ATP concentrations under heat stress, as well as photosystem II activity. This work proves the importance of ATP synthase in cyanobacterial stress acclimation and provides a good target for future improvement of cyanobacterial stress tolerance by metabolic engineering.
关键词	Synechococcus elongatus ATP synthase point mutation abiotic stress photosynthesis
DOI	10.1128/AEM.01222-18
关键词[WOS]	PHOTOSYNTHETIC ELECTRON-TRANSPORT ; SYNECHOCYSTIS SP PCC-6803 ; PHOTOSYSTEM-II ; CYANOBACTERIAL FLAVODOXIN ; CHLOROPHYLL FLUORESCENCE ; ABIOTIC STRESSES ; IRON UPTAKE ; IN-VIVO ; PROTEIN ; LIGHT
语种	英语
资助项目	National Science Fund for Distinguished Young Scholars of China[31525002] ; Shandong Key Basic Research Project ; Chinese Academy of Sciences[ZDRW-ZS-2016-3] ; National Science Foundation of China[31301018] ; National Science Foundation of China[31600034] ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project[15-10-3-15-(31)-zch] ; Scientific Funds for Outstanding Young Scientists of Shandong Province[BS2014HZ003]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
项目资助者	National Science Fund for Distinguished Young Scholars of China ; Shandong Key Basic Research Project ; Chinese Academy of Sciences ; National Science Foundation of China ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project ; Scientific Funds for Outstanding Young Scientists of Shandong Province
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000443291000025
出版者	AMER SOC MICROBIOLOGY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11894
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Lu, Xuefeng
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Freiburg, Germany
推荐引用方式 GB/T 7714	Lou, Wenjing,Tan, Xiaoming,Song, Kuo,et al. A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(18):16.
APA	Lou, Wenjing.,Tan, Xiaoming.,Song, Kuo.,Zhang, Shanshan.,Luan, Guodong.,...&Lu, Xuefeng.(2018).A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(18),16.
MLA	Lou, Wenjing,et al."A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.18(2018):16.