QIBEBT-IR
Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1
Yuan, Cheng2; Xu, Kai1,2; Sun, Juan1; Hu, Guang-Rong2; Li, Fu-2
2018-04-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume30Issue:2Pages:793-801
AbstractProducing valuable coproducts from oleaginous microalgae is an option to reduce the total cost of biofuel production. Here, the influence of nitrogen sources on biomass yield and lipid accumulation of a newly identified oleaginous green microalgal strain, Mychonastes afer HSO-3-1, was evaluated. Carbon assimilation and the following lipid biosynthesis of M. afer were inhibited to some extent under weak acidic conditions (6 < pH < 7) and any of the tested nitrogen source. The highest lipid productivity of 50.7 mg L-1 day(-1) was achieved with a 17.6 mM nitrogen supplement in the form of urea. The cell polar lipid content was significantly higher than triacylglycerol (TAG), and saturated palmitic acid (C16:0) occupied a dominant position in the fatty acid profiles while culturing M. afer in acidic medium with NH4 (+) as the nitrogen source. Under neutral conditions, the lipid productivities of M. afer cultivated in media containing 17.6 mM of NaNO3, NH4Cl, and NH4NO3 were 76.2, 77.5, and 79.0 mg L-1 day(-1), respectively. The greatest TAG content (58.56%) of total lipids was obtained when NaNO3 was used as the nitrogen source. There was no significant difference in the fatty acid composition of M. afer cells when they were cultivated in neutral media supplemented with NaNO3, urea, NH4Cl, and NH4NO3. Therefore, NH4 (+) was not a suitable nitrogen source for M. afer cultivation due to the additional labor, working procedures, and alkali required to adjust the medium pH. Considering that using urea as nitrogen source could reduce the cost of nutrient salts substantially and urea can be taken up and utilized by most microalgae, it is a preferred nitrogen source. The major properties of biodiesel derived from M. afer HSO-3-1 met biodiesel quality, and nervonic acid concentrations remained at approximately 3.0% of total fatty acids.
SubtypeArticle
KeywordChlorophyta Nitrogen Sources Lipid Ph Adjustment Nervonic Acid Mychonastes Afer
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10811-017-1308-y
WOS KeywordARTHROSPIRA SPIRULINA PLATENSIS ; DIATOM NITZSCHIA-LAEVIS ; FED-BATCH CULTIVATION ; NITROGEN-SOURCE ; BIODIESEL PRODUCTION ; TUBULAR PHOTOBIOREACTOR ; CHLORELLA-VULGARIS ; HETEROTROPHIC PRODUCTION ; ALCOHOLIC FERMENTATION ; EICOSAPENTAENOIC ACID
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
Funding OrganizationMinistry of Science and Technology of China(2014AA022003) ; earmarked fund for China Agriculture Research System(CARS-35-33) ; National key research and development program-China(2016YFB0601001) ; Key R&D Program of Shandong Province(2015GSF115027)
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000431194900005
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/11514
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorSun, Juan
Affiliation1.Qingdao Agr Univ, Inst Econ Herb Plants, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Cheng,Xu, Kai,Sun, Juan,et al. Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(2):793-801.
APA Yuan, Cheng,Xu, Kai,Sun, Juan,Hu, Guang-Rong,&Li, Fu-.(2018).Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1.JOURNAL OF APPLIED PHYCOLOGY,30(2),793-801.
MLA Yuan, Cheng,et al."Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1".JOURNAL OF APPLIED PHYCOLOGY 30.2(2018):793-801.
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