QIBEBT-IR
Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1
Yuan, Cheng2; Xu, Kai1,2; Sun, Juan1; Hu, Guang-Rong2; Li, Fu-2
2018-04-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
卷号30期号:2页码:793-801
摘要Producing valuable coproducts from oleaginous microalgae is an option to reduce the total cost of biofuel production. Here, the influence of nitrogen sources on biomass yield and lipid accumulation of a newly identified oleaginous green microalgal strain, Mychonastes afer HSO-3-1, was evaluated. Carbon assimilation and the following lipid biosynthesis of M. afer were inhibited to some extent under weak acidic conditions (6 < pH < 7) and any of the tested nitrogen source. The highest lipid productivity of 50.7 mg L-1 day(-1) was achieved with a 17.6 mM nitrogen supplement in the form of urea. The cell polar lipid content was significantly higher than triacylglycerol (TAG), and saturated palmitic acid (C16:0) occupied a dominant position in the fatty acid profiles while culturing M. afer in acidic medium with NH4 (+) as the nitrogen source. Under neutral conditions, the lipid productivities of M. afer cultivated in media containing 17.6 mM of NaNO3, NH4Cl, and NH4NO3 were 76.2, 77.5, and 79.0 mg L-1 day(-1), respectively. The greatest TAG content (58.56%) of total lipids was obtained when NaNO3 was used as the nitrogen source. There was no significant difference in the fatty acid composition of M. afer cells when they were cultivated in neutral media supplemented with NaNO3, urea, NH4Cl, and NH4NO3. Therefore, NH4 (+) was not a suitable nitrogen source for M. afer cultivation due to the additional labor, working procedures, and alkali required to adjust the medium pH. Considering that using urea as nitrogen source could reduce the cost of nutrient salts substantially and urea can be taken up and utilized by most microalgae, it is a preferred nitrogen source. The major properties of biodiesel derived from M. afer HSO-3-1 met biodiesel quality, and nervonic acid concentrations remained at approximately 3.0% of total fatty acids.
文章类型Article
关键词Chlorophyta Nitrogen Sources Lipid Ph Adjustment Nervonic Acid Mychonastes Afer
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10811-017-1308-y
关键词[WOS]ARTHROSPIRA SPIRULINA PLATENSIS ; DIATOM NITZSCHIA-LAEVIS ; FED-BATCH CULTIVATION ; NITROGEN-SOURCE ; BIODIESEL PRODUCTION ; TUBULAR PHOTOBIOREACTOR ; CHLORELLA-VULGARIS ; HETEROTROPHIC PRODUCTION ; ALCOHOLIC FERMENTATION ; EICOSAPENTAENOIC ACID
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
项目资助者Ministry of Science and Technology of China(2014AA022003) ; earmarked fund for China Agriculture Research System(CARS-35-33) ; National key research and development program-China(2016YFB0601001) ; Key R&D Program of Shandong Province(2015GSF115027)
WOS类目Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号WOS:000431194900005
出版者SPRINGER
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/11514
专题中国科学院青岛生物能源与过程研究所
通讯作者Sun, Juan
作者单位1.Qingdao Agr Univ, Inst Econ Herb Plants, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China
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Yuan, Cheng,Xu, Kai,Sun, Juan,et al. Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(2):793-801.
APA Yuan, Cheng,Xu, Kai,Sun, Juan,Hu, Guang-Rong,&Li, Fu-.(2018).Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1.JOURNAL OF APPLIED PHYCOLOGY,30(2),793-801.
MLA Yuan, Cheng,et al."Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1".JOURNAL OF APPLIED PHYCOLOGY 30.2(2018):793-801.
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