| Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli | |
Wang, Xiangwei1,2; Li, Lingling1,3; Zheng, Yanning1,2; Zou, Huibin1; Cao, Yujin1 ; Liu, Hui1; Liu, Wei1; Xian, Mo1
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| 2012-06-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| 卷号 | 114期号:114页码:561-566 |
| 摘要 | This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450(BM3) to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0 mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources. (C) 2012 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Long Chain Hydroxyfatty Acid Escherichia Coli Thioesterase Fatty Acid Hydroxylase Fadd Knocking Out |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2012.02.119 |
| 关键词[WOS] | FATTY-ACIDS ; BACILLUS-MEGATERIUM ; MICROBIAL-PRODUCTION ; OLEIC-ACID ; MONOOXYGENASE ; HYDROXYLATION ; OMEGA ; BIOCATALYSIS ; THIOESTERASE ; RECOMBINANT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000305035900079 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1120 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiangwei,Li, Lingling,Zheng, Yanning,et al. Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2012,114(114):561-566. |
| APA | Wang, Xiangwei.,Li, Lingling.,Zheng, Yanning.,Zou, Huibin.,Cao, Yujin.,...&Xian, Mo.(2012).Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli.BIORESOURCE TECHNOLOGY,114(114),561-566. |
| MLA | Wang, Xiangwei,et al."Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli".BIORESOURCE TECHNOLOGY 114.114(2012):561-566. |
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