The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine

doi:10.1039/c8bm00675j

QIBEBT-IR

	The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine
	Bao, Zixian; Sun, Yue; Rai, Kamal; Peng, Xinying; Wang, Shilu; Nian, Rui; Xian, Mo
	2018-11-01
发表期刊	BIOMATERIALS SCIENCE
ISSN	2047-4830
卷号	6 期号:11 页码:3042-3052
摘要	Collagen has been widely documented as one of the most promising and competitive biomaterials for tissue engineering and medical applications. However, the properties of collagen differ from one source to another. In the present study, type I collagen (COL-I) was extracted and purified from the skins of Japanese sea bass (Lateolabrax japonicus) and Nile tilapia (Oreochromis niloticus). Ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE were performed to characterize both COL-Is. The denaturing temperature of bass collagen (BC) was observed to be 27.2 degrees C, and 35.3 degrees C for tilapia collagen (TC). The content of hydroxyproline was 13.4% in TC, which was similar to that in porcine collagen (PC, 13.6%) and higher than that in BC (10.3%), while the content of cysteine in TC (0.87%) was significantly higher than that in PC (0.04%) and BC (0.35%). After incubation at different temperatures for 9 h, more degraded collagen bands appeared in the BC hydrogel (BCH) group than in the TC hydrogel (TCH) group, indicating that TCH exhibited better thermal stability than BCH. The thermal stabilities of TCH and PC hydrogel (PCH) were similar. The compressive stress of TCH was up to 0.099 MPa, while it was 0.047 MPa for BCH and 0.003 MPa for PCH. These results demonstrated that the content of amino acids (especially hydroxyproline and cysteine) has a synergistic effect on the thermal and mechanical properties of BCH, TCH and PCH, which would be an indicator of the thermal and mechanical properties of collagen hydrogels in future studies.
DOI	10.1039/c8bm00675j
关键词[WOS]	PEPSIN-SOLUBILIZED COLLAGEN ; FISH SCALE COLLAGEN ; ACID-SOLUBLE COLLAGEN ; ROHU LABEO-ROHITA ; FIBRIL FORMATION ; I COLLAGEN ; OREOCHROMIS-NILOTICAS ; PHYSICAL-PROPERTIES ; PAGRUS-MAJOR ; SWIM BLADDER
语种	英语
资助项目	Shandong Provincial Natural Science Foundation, China[ZR2018BC027] ; National Natural Science Foundation of China[21676286] ; Primary Research Development Plan of Shandong Province[2016GSF121006] ; China Postdoctoral Science Foundation[2017M622302]
WOS研究方向	Materials Science
项目资助者	Shandong Provincial Natural Science Foundation, China ; National Natural Science Foundation of China ; Primary Research Development Plan of Shandong Province ; China Postdoctoral Science Foundation
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000448822800023
出版者	ROYAL SOC CHEMISTRY
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11199
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Nian, Rui; Xian, Mo
作者单位	Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Bao, Zixian,Sun, Yue,Rai, Kamal,et al. The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine[J]. BIOMATERIALS SCIENCE,2018,6(11):3042-3052.
APA	Bao, Zixian.,Sun, Yue.,Rai, Kamal.,Peng, Xinying.,Wang, Shilu.,...&Xian, Mo.(2018).The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine.BIOMATERIALS SCIENCE,6(11),3042-3052.
MLA	Bao, Zixian,et al."The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine".BIOMATERIALS SCIENCE 6.11(2018):3042-3052.