KMS Qingdao Institute of Biomass Energy and Bioprocess Technology ,CAS
| Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes | |
| Zhao, Liman1,2; Li, Yanchun3; Pei, Danfeng4; Huang, Qingrong5; Zhang, Hongwei5; Yang, Zechuan1; Li, Fan1; Shi, Tongfei1,2 | |
| 2019-02-01 | |
| Source Publication | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| Volume | 205Pages:167-175 |
| Abstract | Serum stability is a crucial factor for ideal polymeric gene vectors. In this work, a series of serum-tolerant and low-toxicity glycopolymers/poly(ethyleneimine) (PEI) complexes were designed for gene delivery. Atomic transfer radical polymerization (ATRP) was used to synthesize the comb-shaped random copolymers dextran-gpoly(2-dimethylaminoethyl methacrylate-co-2-lactobionamidoethyl methacrylate) (DDrL). Then DDrLs/PEI were investigated for their use as plasmid DNA (pDNA) vectors, which can completely condense the pDNA into nanoparticles. The DDrLs/PEI/pDNA complexes in serum-containing media showed better stability than PEI/ pDNA complexes. in vitro gene transfection studies showed that DDrLs/PEI exhibited a remarkable transfection efficiency enhancement in the presence of serum compared to that in serum-free conditions. Moreover, the transfection level of DDrLs/PEI were two orders of magnitude higher than that of PEI alone in the presence of 30% serum. DDrLs/PEI complexes with galactose enhanced pDNA delivery to hepatocytes, with higher protein expression in ASGPr-presenting HepG2 than in HeLa cells, which lack the receptor. All of the DDrLs/PEI/pDNA complexes had lower cytotoxicity than PEI/pDNA. |
| Keyword | Galactose PEI Gene delivery Serum-tolerant Hepatocyte |
| DOI | 10.1016/j.carbpol.2018.10.036 |
| WOS Keyword | NONSPECIFIC PROTEIN ADSORPTION ; TRANSFECTION EFFICIENCY ; POLY(ETHYLENE GLYCOL) ; TARGETED DELIVERY ; NONVIRAL VECTORS ; HEPATOMA-CELLS ; DNA COMPLEXES ; PLASMID DNA ; KEY ISSUES ; COPOLYMERS |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[51503201] ; National Natural Science Foundation of China[21704109] ; National Natural Science Foundation of China[21674114] ; National Natural Science Foundation of China[51473168] ; National Natural Science Foundation of China[51028301] |
| WOS Research Area | Chemistry ; Polymer Science |
| Funding Organization | National Natural Science Foundation of China |
| WOS Subject | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| WOS ID | WOS:000450093200019 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qibebt.ac.cn/handle/337004/11017 |
| Collection | 中国科学院青岛生物能源与过程研究所 |
| Corresponding Author | Yang, Zechuan; Li, Fan; Shi, Tongfei |
| Affiliation | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Sci & Technol China, Sch Appl Chem & Engn, Hefei 230026, Peoples R China 3.Jilin Univ, Dept Pediat Resp Med, Hosp 1, Changchun 130021, Jilin, Peoples R China 4.Chinese Acad Sci, Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 5.Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA |
| Recommended Citation GB/T 7714 | Zhao, Liman,Li, Yanchun,Pei, Danfeng,et al. Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes[J]. CARBOHYDRATE POLYMERS,2019,205:167-175. |
| APA | Zhao, Liman.,Li, Yanchun.,Pei, Danfeng.,Huang, Qingrong.,Zhang, Hongwei.,...&Shi, Tongfei.(2019).Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes.CARBOHYDRATE POLYMERS,205,167-175. |
| MLA | Zhao, Liman,et al."Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes".CARBOHYDRATE POLYMERS 205(2019):167-175. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment