Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes

doi:10.1016/j.carbpol.2018.10.036

QIBEBT-IR

	Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes
	Zhao, Liman 1,2; Li, Yanchun 3; Pei, Danfeng 4; Huang, Qingrong 5; Zhang, Hongwei 5; Yang, Zechuan 1; Li, Fan 1; Shi, Tongfei 1,2
	2019-02-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	205 页码:167-175
摘要	Serum stability is a crucial factor for ideal polymeric gene vectors. In this work, a series of serum-tolerant and low-toxicity glycopolymers/poly(ethyleneimine) (PEI) complexes were designed for gene delivery. Atomic transfer radical polymerization (ATRP) was used to synthesize the comb-shaped random copolymers dextran-gpoly(2-dimethylaminoethyl methacrylate-co-2-lactobionamidoethyl methacrylate) (DDrL). Then DDrLs/PEI were investigated for their use as plasmid DNA (pDNA) vectors, which can completely condense the pDNA into nanoparticles. The DDrLs/PEI/pDNA complexes in serum-containing media showed better stability than PEI/ pDNA complexes. in vitro gene transfection studies showed that DDrLs/PEI exhibited a remarkable transfection efficiency enhancement in the presence of serum compared to that in serum-free conditions. Moreover, the transfection level of DDrLs/PEI were two orders of magnitude higher than that of PEI alone in the presence of 30% serum. DDrLs/PEI complexes with galactose enhanced pDNA delivery to hepatocytes, with higher protein expression in ASGPr-presenting HepG2 than in HeLa cells, which lack the receptor. All of the DDrLs/PEI/pDNA complexes had lower cytotoxicity than PEI/pDNA.
关键词	Galactose PEI Gene delivery Serum-tolerant Hepatocyte
DOI	10.1016/j.carbpol.2018.10.036
关键词[WOS]	NONSPECIFIC PROTEIN ADSORPTION ; TRANSFECTION EFFICIENCY ; POLY(ETHYLENE GLYCOL) ; TARGETED DELIVERY ; NONVIRAL VECTORS ; HEPATOMA-CELLS ; DNA COMPLEXES ; PLASMID DNA ; KEY ISSUES ; COPOLYMERS
语种	英语
资助项目	National Natural Science Foundation of China[51503201] ; National Natural Science Foundation of China[21704109] ; National Natural Science Foundation of China[21674114] ; National Natural Science Foundation of China[51473168] ; National Natural Science Foundation of China[51028301]
WOS研究方向	Chemistry ; Polymer Science
项目资助者	National Natural Science Foundation of China
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000450093200019
出版者	ELSEVIER SCI LTD
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11017
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Yang, Zechuan; Li, Fan; Shi, Tongfei
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Sci & Technol China, Sch Appl Chem & Engn, Hefei 230026, Peoples R China 3.Jilin Univ, Dept Pediat Resp Med, Hosp 1, Changchun 130021, Jilin, Peoples R China 4.Chinese Acad Sci, Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 5.Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA
推荐引用方式 GB/T 7714	Zhao, Liman,Li, Yanchun,Pei, Danfeng,et al. Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes[J]. CARBOHYDRATE POLYMERS,2019,205:167-175.
APA	Zhao, Liman.,Li, Yanchun.,Pei, Danfeng.,Huang, Qingrong.,Zhang, Hongwei.,...&Shi, Tongfei.(2019).Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes.CARBOHYDRATE POLYMERS,205,167-175.
MLA	Zhao, Liman,et al."Glycopolymers/PEI complexes as serum-tolerant vectors for enhanced gene delivery to hepatocytes".CARBOHYDRATE POLYMERS 205(2019):167-175.