Two-phase Filtering Strategy for Efficient Peptide Identification from Mass Spectrometry

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	Two-phase Filtering Strategy for Efficient Peptide Identification from Mass Spectrometry
	Ng HK; Leong HW; Ning K
	2010
发表期刊	J Proteomics Bioinform.
期号	0
摘要	Peptide identification by tandem mass spectrometry (MS/MS) is one of the most important problems in proteomics. Recent advances in high throughput MS/MS experiments result in huge amount of spectra, and the peptide identification process should keep pace. In this paper, we strive to achieve high accuracy and efficiency for peptide identification with the presence of noise by a two-phase filtering strategy. Our algorithm transforms spectra to high dimensional vectors, and then uses self-organizing map (SOM) and multi-point range query (MPRQ) as very efficient coarse filters to select a number of candidate peptides from database. These candidate peptides are subsequently scored and ranked by an accurate tag-based scoring function S(λ). Experiments showed that our approach is both fast and accurate for peptide identification.; Peptide identification by tandem mass spectrometry (MS/MS) is one of the most important problems in proteomics. Recent advances in high throughput MS/MS experiments result in huge amount of spectra, and the peptide identification process should keep pace. In this paper, we strive to achieve high accuracy and efficiency for peptide identification with the presence of noise by a two-phase filtering strategy. Our algorithm transforms spectra to high dimensional vectors, and then uses self-organizing map (SOM) and multi-point range query (MPRQ) as very efficient coarse filters to select a number of candidate peptides from database. These candidate peptides are subsequently scored and ranked by an accurate tag-based scoring function S(λ). Experiments showed that our approach is both fast and accurate for peptide identification.
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1085
专题	单细胞中心组群
推荐引用方式 GB/T 7714	Ng HK,Leong HW,Ning K. Two-phase Filtering Strategy for Efficient Peptide Identification from Mass Spectrometry[J]. J Proteomics Bioinform.,2010(0).
APA	Ng HK,Leong HW,&Ning K.(2010).Two-phase Filtering Strategy for Efficient Peptide Identification from Mass Spectrometry.J Proteomics Bioinform.(0).
MLA	Ng HK,et al."Two-phase Filtering Strategy for Efficient Peptide Identification from Mass Spectrometry".J Proteomics Bioinform. .0(2010).

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