Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera

doi:10.7717/peerj.4373

QIBEBT-IR

	Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera
	Wang, Xueqin ; Yu, Huahua ; Xing, Ronge ; Chen, Xiaolin ; Liu, Song ; Li, Pengcheng 1
	2018-02-15
发表期刊	PEERJ
ISSN	2167-8359
卷号	6
摘要	Mackerel (Pneumatophorus japonicus) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 degrees C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
文章类型	Article
关键词	Mackerel Viscera Protease Response Surface Methodology Antioxidant Activity
WOS标题词	Science & Technology
DOI	10.7717/peerj.4373
关键词[WOS]	PROCESSING BY-PRODUCTS ; PROTEIN HYDROLYSATE ; GASTROINTESTINAL DIGESTION ; POTENTIAL APPLICATIONS ; BIOACTIVE PROPERTIES ; IN-VITRO ; IDENTIFICATION ; PURIFICATION ; SKIN ; FRACTIONS
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
项目资助者	National Natural Science Foundation of China(41506175) ; Key Research and Development Program of Shandong Province(2017YYSP018) ; Qingdao Science and Technology Project(17-3-3-21-nsh) ; Chinese Academy of Sciences(KFZD-SW-106) ; Science and Technology Development Plan Project of Shandong Province(2016YYSP010)
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000425362200006
出版者	PEERJ INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10650
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera[J]. PEERJ,2018,6.
APA	Wang, Xueqin,Yu, Huahua,Xing, Ronge,Chen, Xiaolin,Liu, Song,&Li, Pengcheng.(2018).Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera.PEERJ,6.
MLA	Wang, Xueqin,et al."Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera".PEERJ 6(2018).