Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans

doi:10.1080/10826068.2017.1350979

QIBEBT-IR > 生物基材料组群

	Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans
	Chen, Guoqiang 1,2; Wang, Jiming 1; Su, Yulong 3; Zhu, Youshuang 4; Zhang, Ge 1,3; Zhao, Hongwei 1; Liu, Haobao 3; Yang, Ying 1; Nian, Rui 1; Zhang, Haibo1 ; Wei, Yuxi 2; Xian, Mo1
	2017
发表期刊	PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN	1082-6068
卷号	47 期号:10 页码:963-969
摘要	Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. First, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with thin-layer chromatography, Fourier transform infrared, and nuclear magnetic resonance. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4g/L Tween 80 for 96hr of cultivation), continuously fermentation was performed. The highest pullulan production and dry biomass was 109 and 125g/L after fermentation for 114hr, respectively. The average productivity was about 1g/L/hr, which was intensively higher than the previous reported. This study would lay foundations for the industrial production of pullulan.
文章类型	Article
关键词	Aureobasidium Pullulans Fermentation Conditions Ftir Nmr Pullulan Tlc
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1080/10826068.2017.1350979
关键词[WOS]	MOLECULAR-WEIGHT PULLULAN ; SUGAR METABOLISM ; TWEEN 80 ; FERMENTATION ; STRAIN ; OPTIMIZATION ; POTATO ; LIPASE ; EXOPOLYSACCHARIDES ; POLYSACCHARIDE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China (NSF)(31400084) ; Natural Science Foundation of Shandong Province(ZR2014CQ008) ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; Youth Innovation Promotion Association (CAS)(2017252)
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000418064000003
出版者	TAYLOR & FRANCIS INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10522
专题	生物基材料组群
通讯作者	Zhang, Haibo; Wei, Yuxi; Xian, Mo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao, Peoples R China 4.Jining Med Univ, Sch Biol Sci, Jining, Peoples R China 5.189 Songling Rd, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guoqiang,Wang, Jiming,Su, Yulong,et al. Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2017,47(10):963-969.
APA	Chen, Guoqiang.,Wang, Jiming.,Su, Yulong.,Zhu, Youshuang.,Zhang, Ge.,...&Xian, Mo.(2017).Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,47(10),963-969.
MLA	Chen, Guoqiang,et al."Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 47.10(2017):963-969.