Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200

doi:10.1007/s00792-016-0822-1

QIBEBT-IR

	Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200
	Zhang, Sheng-Da 1,3,4; Santini, Claire-Lise 2,3,4; Zhang, Wei-Jia 1,3,4; Barbe, Valerie 5; Mangenot, Sophie 5; Guyomar, Charlotte 2,3,4; Garel, Marc 6; Chen, Hai-Tao 1,3,4; Li, Xue-Gong 1,3,4; Yin, Qun-Jian 1,3,4; Zhao, Yuan 7; Armengaud, Jean 8; Gaillard, Jean-Charles 8; Martini, Severine 6; Pradel, Nathalie 6; Vidaud, Claude 8; Alberto, Francois 2,3,4; Medigue, Claudine 9,10,11; Tamburini, Christian 6; Wu, Long-Fei 2,3,4
	2016-05-01
发表期刊	EXTREMOPHILES
ISSN	1431-0651
卷号	20 期号:3 页码:301-310
摘要	Bacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.
文章类型	Article
关键词	Deep-sea Adaptation Bioluminescence Tmao Reductase Hydrostatic Pressure Anaerobic Respiration
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00792-016-0822-1
关键词[WOS]	ESCHERICHIA-COLI ; RESPONSE REGULATOR ; OPERON EXPRESSION ; MARINE BACTERIUM ; PROFUNDUM SS9 ; STRAIN MO-1 ; PRESSURE ; TORR ; ADAPTATION ; SEQUENCE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
项目资助者	Sanya Institute of Deep-Sea Sciences and Engineering(SIDSSE-201307 ; Strategic Priority Research Program(XDB06010203) ; International Partnership for Innovative Team Program from the Chinese Academy of Sciences(20140491526) ; National Natural Science Foundation of China(NSFC 41506147) ; Centre National de la Recherche Scientifique ; China Ocean Mineral Resources R & D Association (COMRA) Special Foundation(DY125-15-R-03) ; National Science Foundation of China(NSFC 41306161) ; Mt. Tai Scholar Construction Engineering Special Foundation of Shandong Province ; France Genomique ; SIDSSE-QN-201405 ; SIDSSE-QN-201406 ; SIDSSE-QN-201408)
WOS类目	Biochemistry & Molecular Biology ; Microbiology
WOS记录号	WOS:000374832100007
出版者	SPRINGER JAPAN KK
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10452
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wu, Long-Fei
作者单位	1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Deep Sea Microbial Cell Biol, Sanya, Peoples R China 2.Aix Marseille Univ, CNRS, IMM, LCB UMR 7257, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France 3.LCB CNRS, France China Biomineralizat & Nanostruct Lab, LIA BioMNSL, Marseille, France 4.Chinese Acad Sci, SIDSSE, Sanya, Peoples R China 5.CEA, DSV IG Genoscope LF, Evry, France 6.Univ Sud Toulon Var, Aix Marseille Univ, CNRS INSU, IRD,MIO,UM110, F-13288 Marseille, France 7.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 8.CEA, DSV, IBEB, SBTN, Bagnols Sur Ceze, France 9.CEA, DSV IG Genoscope, Lab Analyse Bioinformat Genom & Metab, Evry, France 10.CNRS, UMR 8030, Evry, France 11.Univ Evry Val Esssone, Evry, France
推荐引用方式 GB/T 7714	Zhang, Sheng-Da,Santini, Claire-Lise,Zhang, Wei-Jia,et al. Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200[J]. EXTREMOPHILES,2016,20(3):301-310.
APA	Zhang, Sheng-Da.,Santini, Claire-Lise.,Zhang, Wei-Jia.,Barbe, Valerie.,Mangenot, Sophie.,...&Wu, Long-Fei.(2016).Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.EXTREMOPHILES,20(3),301-310.
MLA	Zhang, Sheng-Da,et al."Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200".EXTREMOPHILES 20.3(2016):301-310.