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Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942
Qiao, Cuncun1,2,5; Duan, Yangkai1,3; Zhang, Mingyi1,3,5; Hagemann, Martin6; Luo, Quan1,3; Lu, Xuefeng1,4
2018
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume84Issue:2
AbstractSucrose and glycogen syntheses in cyanobacteria share the common precursor glucose-1-phosphate. It is generally assumed that lowering glycogen synthesis could drive more carbon toward sucrose synthesis that can be induced by salt stress among cyanobacteria. By using a theophylline-dependent riboswitch system, the expression of glgC, a key gene in glycogen synthesis, was downregulated in a quantitative manner in a sucrose-secreting strain of Synechococcus elongatus PCC 7942. We observed that the stepwise suppression of glycogen synthesis limited rather than stimulated sucrose production in the salt-stressed cells, suggesting that glycogen could serve as a carbon pool for the synthesis of sucrose. Accordingly, we generated glycogen-overproducing strains, but the increased glycogen pool alone did not stimulate sucrose production, indicating that alternative steps limit the carbon flux toward the synthesis of sucrose. Consistent with previous studies that showed that sucrose-phosphate synthase (SPS) catalyzes the rate-limiting step in sucrose synthesis, the combination of glycogen overproduction and sps overexpression resulted in increased sucrose production. Our results indicate that the glycogen and sucrose pools are closely linked in Synechococcus elongatus PCC 7942, and we propose that enhancing the glycogen pool could be a promising strategy for the improvement of sucrose production by cyanobacteria in the presence of a strong sucrose synthesis sink.
SubtypeArticle
KeywordSucrose Production Glycogen Riboswitch Synechococcus Elongatus Pcc 7942
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1128/AEM.02023-17
WOS KeywordPHOTOSYNTHETIC PRODUCTION ; STRESS RESPONSES ; CYANOBACTERIUM ; MUTANTS ; BIOSYNTHESIS ; CHEMICALS ; TURNOVER ; FUELS
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
Funding OrganizationNational Science Fund for Distinguished Young Scholars of China(31525002) ; joint Sino-German research project(GZ984) ; National Science Foundation of China(31501003) ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project(15-10-3-15-(31)-zch)
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000419048900015
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10016
Collection微生物代谢工程团队
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol Lab, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Univ Rostock, Inst Biowissensch, Pflanzenphysiol, Rostock, Germany
Recommended Citation
GB/T 7714
Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(2).
APA Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,Hagemann, Martin,Luo, Quan,&Lu, Xuefeng.(2018).Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(2).
MLA Qiao, Cuncun,et al."Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.2(2018).
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