中国科学院青岛生物能源与过程研究所机构知识库
Advanced  
QIBEBT-IR  > 微生物代谢工程团队  > 期刊论文
Title:
Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942
Author: Qiao, Cuncun1,2,5; Duan, Yangkai1,3; Zhang, Mingyi1,3,5; Hagemann, Martin6; Luo, Quan1,3; Lu, Xuefeng1,4
Source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Issued Date: 2018
Volume: 84, Issue:2
Keyword: sucrose production ; glycogen ; riboswitch ; Synechococcus elongatus PCC 7942
DOI: 10.1128/AEM.02023-17
DOC Type: Article
English Abstract: Sucrose and glycogen syntheses in cyanobacteria share the common precursor glucose-1-phosphate. It is generally assumed that lowering glycogen synthesis could drive more carbon toward sucrose synthesis that can be induced by salt stress among cyanobacteria. By using a theophylline-dependent riboswitch system, the expression of glgC, a key gene in glycogen synthesis, was downregulated in a quantitative manner in a sucrose-secreting strain of Synechococcus elongatus PCC 7942. We observed that the stepwise suppression of glycogen synthesis limited rather than stimulated sucrose production in the salt-stressed cells, suggesting that glycogen could serve as a carbon pool for the synthesis of sucrose. Accordingly, we generated glycogen-overproducing strains, but the increased glycogen pool alone did not stimulate sucrose production, indicating that alternative steps limit the carbon flux toward the synthesis of sucrose. Consistent with previous studies that showed that sucrose-phosphate synthase (SPS) catalyzes the rate-limiting step in sucrose synthesis, the combination of glycogen overproduction and sps overexpression resulted in increased sucrose production. Our results indicate that the glycogen and sucrose pools are closely linked in Synechococcus elongatus PCC 7942, and we propose that enhancing the glycogen pool could be a promising strategy for the improvement of sucrose production by cyanobacteria in the presence of a strong sucrose synthesis sink.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biotechnology & Applied Microbiology ; Microbiology
WOS Subject Extended: Biotechnology & Applied Microbiology ; Microbiology
WOS Keyword Plus: PHOTOSYNTHETIC PRODUCTION ; STRESS RESPONSES ; CYANOBACTERIUM ; MUTANTS ; BIOSYNTHESIS ; CHEMICALS ; TURNOVER ; FUELS
Indexed Type: SCI
Funder: National Science Fund for Distinguished Young Scholars of China(31525002) ; joint Sino-German research project(GZ984) ; National Science Foundation of China(31501003) ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project(15-10-3-15-(31)-zch)
Language: 英语
WOS ID: WOS:000419048900015
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/10016
Appears in Collections:微生物代谢工程团队_期刊论文

Files in This Item:

There are no files associated with this item.


description.institution: 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol Lab, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Univ Rostock, Inst Biowissensch, Pflanzenphysiol, Rostock, Germany

Recommended Citation:
Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(2).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qiao, Cuncun]'s Articles
[Duan, Yangkai]'s Articles
[Zhang, Mingyi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qiao, Cuncun]‘s Articles
[Duan, Yangkai]‘s Articles
[Zhang, Mingyi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace