QIBEBT-IR  > 微生物代谢工程团队
Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942
Qiao, Cuncun1,2,5; Duan, Yangkai1,3; Zhang, Mingyi1,3,5; Hagemann, Martin6; Luo, Quan1,3; Lu, Xuefeng1,4
2018
发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号84期号:2
摘要Sucrose and glycogen syntheses in cyanobacteria share the common precursor glucose-1-phosphate. It is generally assumed that lowering glycogen synthesis could drive more carbon toward sucrose synthesis that can be induced by salt stress among cyanobacteria. By using a theophylline-dependent riboswitch system, the expression of glgC, a key gene in glycogen synthesis, was downregulated in a quantitative manner in a sucrose-secreting strain of Synechococcus elongatus PCC 7942. We observed that the stepwise suppression of glycogen synthesis limited rather than stimulated sucrose production in the salt-stressed cells, suggesting that glycogen could serve as a carbon pool for the synthesis of sucrose. Accordingly, we generated glycogen-overproducing strains, but the increased glycogen pool alone did not stimulate sucrose production, indicating that alternative steps limit the carbon flux toward the synthesis of sucrose. Consistent with previous studies that showed that sucrose-phosphate synthase (SPS) catalyzes the rate-limiting step in sucrose synthesis, the combination of glycogen overproduction and sps overexpression resulted in increased sucrose production. Our results indicate that the glycogen and sucrose pools are closely linked in Synechococcus elongatus PCC 7942, and we propose that enhancing the glycogen pool could be a promising strategy for the improvement of sucrose production by cyanobacteria in the presence of a strong sucrose synthesis sink.
文章类型Article
关键词Sucrose Production Glycogen Riboswitch Synechococcus Elongatus Pcc 7942
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1128/AEM.02023-17
关键词[WOS]PHOTOSYNTHETIC PRODUCTION ; STRESS RESPONSES ; CYANOBACTERIUM ; MUTANTS ; BIOSYNTHESIS ; CHEMICALS ; TURNOVER ; FUELS
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
项目资助者National Science Fund for Distinguished Young Scholars of China(31525002) ; joint Sino-German research project(GZ984) ; National Science Foundation of China(31501003) ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project(15-10-3-15-(31)-zch)
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000419048900015
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/10016
专题微生物代谢工程团队
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol Lab, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Univ Rostock, Inst Biowissensch, Pflanzenphysiol, Rostock, Germany
推荐引用方式
GB/T 7714
Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(2).
APA Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,Hagemann, Martin,Luo, Quan,&Lu, Xuefeng.(2018).Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(2).
MLA Qiao, Cuncun,et al."Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.2(2018).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qiao, Cuncun]的文章
[Duan, Yangkai]的文章
[Zhang, Mingyi]的文章
百度学术
百度学术中相似的文章
[Qiao, Cuncun]的文章
[Duan, Yangkai]的文章
[Zhang, Mingyi]的文章
必应学术
必应学术中相似的文章
[Qiao, Cuncun]的文章
[Duan, Yangkai]的文章
[Zhang, Mingyi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。