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Microbial production of mevalonate byrecombinant Escherichia coli using acetic acid as a carbon source
Xin Xu, Meng Xie, Qian Zhao, Mo Xian & Huizhou Liu; Huibin Zou; Mo Xian.
2017-06-02
Source PublicationBioengineered
Volume20Pages:33-41
AbstractWe sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06 g L¡1 mevalonate with a productivity of 0.03 g L¡1 h¡1 in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85 g L¡1 and 0.13 g L¡1 h¡1, respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05 g L¡1 and 4.97 g L¡1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
SubtypeArticle
KeywordAcetate Assimilation Aeration Mevalonate Two-stage Fed-batch Escherichia Coli
DOI10.1080/21655979.2017.1323592
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9913
Collection材料生物技术研究中心
Corresponding AuthorHuibin Zou; Mo Xian.
Affiliation中国科学院广州能源所
Recommended Citation
GB/T 7714
Xin Xu, Meng Xie, Qian Zhao, Mo Xian & Huizhou Liu,Huibin Zou,Mo Xian.. Microbial production of mevalonate byrecombinant Escherichia coli using acetic acid as a carbon source[J]. Bioengineered,2017,20:33-41.
APA Xin Xu, Meng Xie, Qian Zhao, Mo Xian & Huizhou Liu,Huibin Zou,&Mo Xian..(2017).Microbial production of mevalonate byrecombinant Escherichia coli using acetic acid as a carbon source.Bioengineered,20,33-41.
MLA Xin Xu, Meng Xie, Qian Zhao, Mo Xian & Huizhou Liu,et al."Microbial production of mevalonate byrecombinant Escherichia coli using acetic acid as a carbon source".Bioengineered 20(2017):33-41.
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