A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.

doi:10.1080/21655979.2017.1286432

QIBEBT-IR > 生物基材料组群

	A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.
	Stephen T Lacmata, Lan Yao, Mo Xian, Hui Liu, Jules-Roger Kuiate, Huizhou Liu, Xinjun Feng, Guang Zhao.; Xinjun Feng; Guang Zhao.
	2017-01-19
发表期刊	Bioengineered
卷号	8 期号:5 页码:594-599
摘要	The release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli. The autolysis system was constructed by inserting the 50untranslated region (50UTR) behind promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into one plasmid, the P3HP content of 72.7% (2.4 times of the control) and the plasmid stability of 79.8 § 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system activated in Mg2C-depleted conditions proves to be feasible for polyhydroxyalkanoates production, which may have great application potential for other intracellular products.
文章类型	Article
关键词	Autolysis System Industrial applications Magnesium P3hp Promoter Untranslated Regions
DOI	10.1080/21655979.2017.1286432
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9911
专题	生物基材料组群
通讯作者	Xinjun Feng; Guang Zhao.
作者单位	中国科学院青岛生物能源与过程研究所(2)喀麦隆杜昌大学
推荐引用方式 GB/T 7714	Stephen T Lacmata, Lan Yao, Mo Xian, Hui Liu, Jules-Roger Kuiate, Huizhou Liu, Xinjun Feng, Guang Zhao.,Xinjun Feng,Guang Zhao.. A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.[J]. Bioengineered,2017,8(5):594-599.
APA	Stephen T Lacmata, Lan Yao, Mo Xian, Hui Liu, Jules-Roger Kuiate, Huizhou Liu, Xinjun Feng, Guang Zhao.,Xinjun Feng,&Guang Zhao..(2017).A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli..Bioengineered,8(5),594-599.
MLA	Stephen T Lacmata, Lan Yao, Mo Xian, Hui Liu, Jules-Roger Kuiate, Huizhou Liu, Xinjun Feng, Guang Zhao.,et al."A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.".Bioengineered 8.5(2017):594-599.

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