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Production of D-lactate from glucose using Klebsiella pneumoniae mutants.
Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.; Huibin Zou; Guang Zhao; Mo Xian.
2017
Source PublicationMicrobial Cell Factories
Volume16Pages:209-230
AbstractBackground: d-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for d-lactate production with a signifcant amount of by-products, which complicated the purifcation process and decreased the yield of d-lactate. Results: Based on the redirection of carbon towards by-product formation, the efects of single-gene and multiplegene deletions in K. pneumoniae on d-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had diferent production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure d-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L d-lactate with a yield of 0.91 g/g glucose at 2.5 vvm. Conclusions: Knocking out by-product synthesis genes had a remarkable infuence on the production and yield of d-lactate. This study demonstrated, for the frst time, that K. pneumoniae has great potential to convert monosaccharides into d-lactate. The results provide new insights for industrial production of d-lactate by K. pneumoniae.
SubtypeArticle
KeywordD-lactate Acetolactate Synthase Acetate Kinase Alcohol Dehydrogenase Klebsiella Pneumoniae
DOI10.1186/s12934-017-0822-6
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Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9910
Collection生物基材料组群
Corresponding AuthorHuibin Zou; Guang Zhao; Mo Xian.
Affiliation中国科学院青岛生物能源与过程研究所(2)中国科学院广州能源所(3)中国科学院大学
Recommended Citation
GB/T 7714
Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,Huibin Zou,Guang Zhao,et al. Production of D-lactate from glucose using Klebsiella pneumoniae mutants.[J]. Microbial Cell Factories,2017,16:209-230.
APA Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,Huibin Zou,Guang Zhao,&Mo Xian..(2017).Production of D-lactate from glucose using Klebsiella pneumoniae mutants..Microbial Cell Factories,16,209-230.
MLA Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,et al."Production of D-lactate from glucose using Klebsiella pneumoniae mutants.".Microbial Cell Factories 16(2017):209-230.
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