|Production of D-lactate from glucose using Klebsiella pneumoniae mutants.|
|Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.; Huibin Zou; Guang Zhao; Mo Xian.
|Source Publication||Microbial Cell Factories
|Abstract||Background: d-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for d-lactate production with a signifcant amount of
by-products, which complicated the purifcation process and decreased the yield of d-lactate.
Results: Based on the redirection of carbon towards by-product formation, the efects of single-gene and multiplegene deletions in K. pneumoniae on d-lactate production from glucose via acetolactate synthase (budB), acetate
kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had diferent production
behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had
the most powerful ability to produce optically pure d-lactate from glucose, and was tested with xylose and arabinose
as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L d-lactate with a yield of 0.91 g/g glucose at 2.5 vvm.
Conclusions: Knocking out by-product synthesis genes had a remarkable infuence on the production and yield of
d-lactate. This study demonstrated, for the frst time, that K. pneumoniae has great potential to convert monosaccharides into d-lactate. The results provide new insights for industrial production of d-lactate by K. pneumoniae.|
|Corresponding Author||Huibin Zou; Guang Zhao; Mo Xian.|
Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,Huibin Zou,Guang Zhao,et al. Production of D-lactate from glucose using Klebsiella pneumoniae mutants.[J]. Microbial Cell Factories,2017,16:209-230.
Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,Huibin Zou,Guang Zhao,&Mo Xian..(2017).Production of D-lactate from glucose using Klebsiella pneumoniae mutants..Microbial Cell Factories,16,209-230.
Xinjun Feng, Liqun Jiang, Xiaojuan Han, Xiutao Liu, Zhiqiang Zhao, Huizhou Liu, Mo Xian, Guang Zhao.,et al."Production of D-lactate from glucose using Klebsiella pneumoniae mutants.".Microbial Cell Factories 16(2017):209-230.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.