Enzymatic process optimization for the in vitro production of isoprene from mevalonate.

doi:10.1186/s12934-016-0622-4

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	Enzymatic process optimization for the in vitro production of isoprene from mevalonate.
	Tao Cheng; Hui Liu; Huibin Zou; Ningning Chen; Mengxun Shi; Congxia Xie; , Guang Zhao; and Mo Xian; Guang Zhao; Mo Xian.
	2017
发表期刊	Microbial Cell Factories
卷号	16 页码:8-16
摘要	Background: As an important bulk chemical for synthetic rubber, isoprene can be biosynthesized by robust microbes. But rational engineering and optimization are often demanded to make the in vivo process feasible due to the complexities of cellular metabolism. Alternative synthetic biochemistry strategies are in fast development to produce isoprene or isoprenoids in vitro. Results: This study set up an in vitro enzyme synthetic chemistry process using 5 enzymes in the lower mevalonate pathway to produce isoprene from mevalonate. We found the level and ratio of individual enzymes would signifcantly aﬀect the efciency of the whole system. The optimized process using 10 balanced enzyme unites (5.0 µM of MVK, PMK, MVD; 10.0 µM of IDI, 80.0 µM of ISPS) could produce 6323.5 µmol/L/h (430 mg/L/h) isoprene in a 2 ml in vitro system. In a scale up process (50 ml) only using 1 balanced enzyme unit (0.5 µM of MVK, PMK, MVD; 1.0 µM of IDI, 8.0 µM of ISPS), the system could produce 302 mg/L isoprene in 40 h, which showed higher production rate and longer reaction phase with comparison of the in vivo control. Conclusions: By optimizing the enzyme levels of lower MVA pathway, synthetic biochemistry methods could be set up for the enzymatic production of isoprene or isoprenoids from mevalonate.
文章类型	Article
关键词	Mevalonate Isoprene Synthetic Biochemistry Isoprenoids Bio-based Chemicals Enzymatic Process
DOI	10.1186/s12934-016-0622-4
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9909
专题	生物基材料组群
通讯作者	Huibin Zou; Guang Zhao; Mo Xian.
作者单位	中国科学院青岛生物能源与过程研究所(2)青岛科技大学
推荐引用方式 GB/T 7714	Tao Cheng,Hui Liu,Huibin Zou,et al. Enzymatic process optimization for the in vitro production of isoprene from mevalonate.[J]. Microbial Cell Factories,2017,16:8-16.
APA	Tao Cheng.,Hui Liu.,Huibin Zou.,Ningning Chen.,Mengxun Shi.,...&Mo Xian..(2017).Enzymatic process optimization for the in vitro production of isoprene from mevalonate..Microbial Cell Factories,16,8-16.
MLA	Tao Cheng,et al."Enzymatic process optimization for the in vitro production of isoprene from mevalonate.".Microbial Cell Factories 16(2017):8-16.

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