|Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.|
|Min Liu; Yamei Ding; Hailin Chen; Zhe Zhao; Huizhou Liu; Mo Xian; Guang Zhao.; Guang Zhao
|Source Publication||BMC Microbiology
|Abstract||Background: Acetyl-CoA-derived chemicals are suitable for multiple applications in many industries. The bio-production
of these chemicals has become imperative owing to the economic and environmental problems. However, acetate
overflow is the major drawback for acetyl-CoA-derived chemicals production. Approaches for overcoming acetate
overflow may be beneficial for the production of acetyl-CoA-derived chemicals.
Results: In this study, a transcriptional regulator iclR was knocked out in E.coli BL21(DE3) to overcome acetate overflow
and improve the chemicals production. Two important acetyl-CoA-derived chemicals, phloroglucinol (PG) and
3-hydroxypropionate (3HP) were used to evaluate it. It is revealed that knockout of iclR significantly increased
expressions of aceBAK operon. The cell yields and glucose utilization efficiencies were higher than those of control
strains. The acetate concentrations were decreased by more than 50% and the productions of PG and 3HP were
increased more than twice in iclR mutants. The effects of iclR knockout on cell physiology, cell metabolism and
production of acetyl-CoA-derived chemicals were similar to those of arcA knockout in our previous study. However, the
arcA-iclR double mutants couldn’t gain higher productions of PG and 3HP. The mechanisms are unclear and needed to
be resolved in future.
Conclusions: Knockout of iclR significantly increased gene expression of aceBAK operon and concomitantly activated
glyoxylate pathway. This genetic modification may be a good way to overcome acetate overflow, and improve the
production of a wide range of acetyl-CoA-derived chemicals.|
|Corresponding Author||Mo Xian; Guang Zhao|
Min Liu,Yamei Ding,Hailin Chen,et al. Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.[J]. BMC Microbiology,2017,17:10-19.
Min Liu.,Yamei Ding.,Hailin Chen.,Zhe Zhao.,Huizhou Liu.,...&Guang Zhao.(2017).Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion..BMC Microbiology,17,10-19.
Min Liu,et al."Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.".BMC Microbiology 17(2017):10-19.
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