QIBEBT-IR  > 生物基材料组群
Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.
Min Liu; Yamei Ding; Hailin Chen; Zhe Zhao; Huizhou Liu; Mo Xian; Guang Zhao.; Guang Zhao
2017-01-07
发表期刊BMC Microbiology
卷号17页码:10-19
摘要Background: Acetyl-CoA-derived chemicals are suitable for multiple applications in many industries. The bio-production of these chemicals has become imperative owing to the economic and environmental problems. However, acetate overflow is the major drawback for acetyl-CoA-derived chemicals production. Approaches for overcoming acetate overflow may be beneficial for the production of acetyl-CoA-derived chemicals. Results: In this study, a transcriptional regulator iclR was knocked out in E.coli BL21(DE3) to overcome acetate overflow and improve the chemicals production. Two important acetyl-CoA-derived chemicals, phloroglucinol (PG) and 3-hydroxypropionate (3HP) were used to evaluate it. It is revealed that knockout of iclR significantly increased expressions of aceBAK operon. The cell yields and glucose utilization efficiencies were higher than those of control strains. The acetate concentrations were decreased by more than 50% and the productions of PG and 3HP were increased more than twice in iclR mutants. The effects of iclR knockout on cell physiology, cell metabolism and production of acetyl-CoA-derived chemicals were similar to those of arcA knockout in our previous study. However, the arcA-iclR double mutants couldn’t gain higher productions of PG and 3HP. The mechanisms are unclear and needed to be resolved in future. Conclusions: Knockout of iclR significantly increased gene expression of aceBAK operon and concomitantly activated glyoxylate pathway. This genetic modification may be a good way to overcome acetate overflow, and improve the production of a wide range of acetyl-CoA-derived chemicals.
文章类型Article
关键词Acetyl-coa Escherichia Coli Iclr Arca 3-hydroxypropionate Phloroglucinol
DOI10.1186/s12866-016-0913-2
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9907
专题生物基材料组群
通讯作者Mo Xian; Guang Zhao
作者单位中国科学院青岛生物能源与过程研究所(2)中国科学院大学(3)中国科学院海洋研究所
推荐引用方式
GB/T 7714
Min Liu,Yamei Ding,Hailin Chen,et al. Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.[J]. BMC Microbiology,2017,17:10-19.
APA Min Liu.,Yamei Ding.,Hailin Chen.,Zhe Zhao.,Huizhou Liu.,...&Guang Zhao.(2017).Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion..BMC Microbiology,17,10-19.
MLA Min Liu,et al."Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.".BMC Microbiology 17(2017):10-19.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
刘敏Improving the prod(945KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Min Liu]的文章
[Yamei Ding]的文章
[Hailin Chen]的文章
百度学术
百度学术中相似的文章
[Min Liu]的文章
[Yamei Ding]的文章
[Hailin Chen]的文章
必应学术
必应学术中相似的文章
[Min Liu]的文章
[Yamei Ding]的文章
[Hailin Chen]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 刘敏Improving the production of acetyl-CoA-derived chemicals in.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。