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Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.
Min Liu; Yamei Ding; Hailin Chen; Zhe Zhao; Huizhou Liu; Mo Xian; Guang Zhao.; Guang Zhao
2017-01-07
Source PublicationBMC Microbiology
Volume17Pages:10-19
AbstractBackground: Acetyl-CoA-derived chemicals are suitable for multiple applications in many industries. The bio-production of these chemicals has become imperative owing to the economic and environmental problems. However, acetate overflow is the major drawback for acetyl-CoA-derived chemicals production. Approaches for overcoming acetate overflow may be beneficial for the production of acetyl-CoA-derived chemicals. Results: In this study, a transcriptional regulator iclR was knocked out in E.coli BL21(DE3) to overcome acetate overflow and improve the chemicals production. Two important acetyl-CoA-derived chemicals, phloroglucinol (PG) and 3-hydroxypropionate (3HP) were used to evaluate it. It is revealed that knockout of iclR significantly increased expressions of aceBAK operon. The cell yields and glucose utilization efficiencies were higher than those of control strains. The acetate concentrations were decreased by more than 50% and the productions of PG and 3HP were increased more than twice in iclR mutants. The effects of iclR knockout on cell physiology, cell metabolism and production of acetyl-CoA-derived chemicals were similar to those of arcA knockout in our previous study. However, the arcA-iclR double mutants couldn’t gain higher productions of PG and 3HP. The mechanisms are unclear and needed to be resolved in future. Conclusions: Knockout of iclR significantly increased gene expression of aceBAK operon and concomitantly activated glyoxylate pathway. This genetic modification may be a good way to overcome acetate overflow, and improve the production of a wide range of acetyl-CoA-derived chemicals.
SubtypeArticle
KeywordAcetyl-coa Escherichia Coli Iclr Arca 3-hydroxypropionate Phloroglucinol
DOI10.1186/s12866-016-0913-2
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Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9907
Collection生物基材料组群
Corresponding AuthorMo Xian; Guang Zhao
Affiliation中国科学院青岛生物能源与过程研究所(2)中国科学院大学(3)中国科学院海洋研究所
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Min Liu,Yamei Ding,Hailin Chen,et al. Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.[J]. BMC Microbiology,2017,17:10-19.
APA Min Liu.,Yamei Ding.,Hailin Chen.,Zhe Zhao.,Huizhou Liu.,...&Guang Zhao.(2017).Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion..BMC Microbiology,17,10-19.
MLA Min Liu,et al."Improving the production of acetyl-CoA-derived chemicals in Escherichia coliBL21(DE3) through iclR and arcA deletion.".BMC Microbiology 17(2017):10-19.
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