High titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.

doi:https://doi.org/10.1016/j.procbio.2017.07.021

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	High titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.
	Hui Liu, Tao Cheng, Huibin Zou, Haibo Zhang, Xin Xu, Chao Sun, Elhussiny Aboulnaga, Zhongkai Cheng, Guang Zhao, Mo Xian
	2017
发表期刊	Process Biochemistry
卷号	62 页码:1-9
摘要	Isoprenoids are important fine chemicals as material monomers, advanced fuels and pharmaceuticals. A variety of natural isoprenoids can be synthesized by engineered microbial strains. This work established a process by dividing the current isoprenoid pathway into the upstream fermentation process, from sugar to mevalonate (MVA), and the downstream process, from MVA to the target isoprenoids. The results showed that significant differences existed in the process conditions between the upstream and downstream fermentations. After individually optimizing the process conditions, the upstream MVA production (84.0 g/L, 34.0% and 1.8 g/ L/h) and downstream isoprene production (11.0 g/L and 0.23 g/L/h) were greatly improved in this two-step process. Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab). Therefore, the two-step route proposed in this study may have potential benefits towards the current isoprenoids production directly from glucose. The high titer and yield of MVA indicate that MVA has great potential to be more broadly utilized as starting precursor in synthetic biology.
DOI	https://doi.org/10.1016/j.procbio.2017.07.021
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9906
专题	生物基材料组群
作者单位	中国科学院青岛生物能源与过程研究所(2)青岛科技大学 (3)Mansoura University
推荐引用方式 GB/T 7714	Hui Liu, Tao Cheng, Huibin Zou, Haibo Zhang, Xin Xu, Chao Sun, Elhussiny Aboulnaga, Zhongkai Cheng, Guang Zhao, Mo Xian. High titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.[J]. Process Biochemistry,2017,62:1-9.
APA	Hui Liu, Tao Cheng, Huibin Zou, Haibo Zhang, Xin Xu, Chao Sun, Elhussiny Aboulnaga, Zhongkai Cheng, Guang Zhao, Mo Xian.(2017).High titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli..Process Biochemistry,62,1-9.
MLA	Hui Liu, Tao Cheng, Huibin Zou, Haibo Zhang, Xin Xu, Chao Sun, Elhussiny Aboulnaga, Zhongkai Cheng, Guang Zhao, Mo Xian."High titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.".Process Biochemistry 62(2017):1-9.

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