| Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation | |
| Danxiang Han, Jing Jia, Jing Li, Milton Sommerfeld, Jian Xu, and Qiang Hu; Danxiang Han | |
| 2017-08-04 | |
| 发表期刊 | Frontier Marine Sci
 |
| 卷号 | 7期号:7页码:7206 |
| 摘要 | An electrospray ionization mass spectrometry-based lipidomics method was developed and integrated with transcriptomics to elucidate metabolic remodeling and turnover of microalgal membrane lipids by using Nannochloropsis oceanica as a model. The lack of lipidome analytical tools has limited our ability to gain new knowledge about lipid metabolism in microalgae, especially for membrane glycerolipids. An electrospray ionization mass spectrometry-based lipidomics method was developed for Nannochloropsis oceanica IMET1, which resolved 41 membrane glycerolipids molecular species belonging to eight classes. Changes in membrane glycerolipids under nitrogen deprivation and high-light (HL) conditions were uncovered. The results showed that the amount of plastidial membrane lipids including monogalactosyldiacylglycerol, phosphatidylglycerol, and the extraplastidic lipids diacylglyceryl-O-4′-(N, N, N,-trimethyl) homoserine and phosphatidylcholine decreased drastically under HL and nitrogen deprivation stresses. Algal cells accumulated considerably more digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerols under stresses. The genes encoding enzymes responsible for biosynthesis, modification and degradation of glycerolipids were identified by mining a time-course global RNA-seq data set. It suggested that reduction in lipid contents under nitrogen deprivation is not attributable to the retarded biosynthesis processes, at least at the gene expression level, as most genes involved in their biosynthesis were unaffected by nitrogen supply, yet several genes were significantly up-regulated. Additionally, a conceptual eicosapentaenoic acid (EPA) biosynthesis network is proposed based on the lipidomic and transcriptomic data, which underlined import of EPA from cytosolic glycerolipids to the plastid for synthesizing EPA-containing chloroplast membrane lipids. |
| 文章类型 | Article |
| 学科领域 | 农林学1区 |
| DOI | 10.3389/fmars.2017.00242 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | State Development & Investment Corporation (SDIC) of China |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9866 |
| 专题 | 单细胞中心组群 |
| 通讯作者 | Danxiang Han |
| 推荐引用方式 GB/T 7714 | Danxiang Han, Jing Jia, Jing Li, Milton Sommerfeld, Jian Xu, and Qiang Hu,Danxiang Han. Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation[J]. Frontier Marine Sci,2017,7(7):7206. |
| APA | Danxiang Han, Jing Jia, Jing Li, Milton Sommerfeld, Jian Xu, and Qiang Hu,&Danxiang Han.(2017).Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation.Frontier Marine Sci,7(7),7206. |
| MLA | Danxiang Han, Jing Jia, Jing Li, Milton Sommerfeld, Jian Xu, and Qiang Hu,et al."Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation".Frontier Marine Sci 7.7(2017):7206. |
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| Metabolic Remodeling（3113KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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