Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases | |
Xin, Yi1,2,10; Lu, Yandu1,2,10; Lee, Yi-Ying3,4; Wei, Li1,2,10; Jia, Jing1,2,10; Wang, Qintao1,2,10; Wang, Dongmei1,2,10; Bai, Fali9,10; Hu, Hanhua8,10; Hu, Qiang7,10; Liu, Jin3,4,5,6; Li, Yantao3,4; Xu, Jian1,2,10; Xu J(徐健) | |
2017-12-04 | |
发表期刊 | MOLECULAR PLANT |
卷号 | 10期号:12页码:1523-1539 |
摘要 | Microalgal oils, depending on their degree of unsaturation, can be utilized as either nutritional supplements or fuels; thus, a feedstock with genetically designed and tunable degree of unsaturation is desirable to maximize process efficiency and product versatility. Systematic profiling of ex vivo (in yeast), in vitro, and in vivo activities of type-2 diacylglycerol acyltransferases in Nannochloropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturated fatty acids (SFAs), NoDGAT2D prefers monounsaturated fatty acids (MUFAs), and NoDGAT2C exhibits the strongest activity toward polyunsaturated fatty acids (PUFAs). As NoDGAT2A, 2C, and 2D originated from the green alga, red alga, and eukaryotic host ancestral participants of secondary endosymbiosis, respectively, a mechanistic model of oleaginousness was unveiled, in which the indigenous and adopted NoDGAT2s formulated functional complementarity and specific transcript abundance ratio that underlie a rigid SFA: MUFA:PUFA hierarchy in triacylglycerol (TAG). By rationally modulating the ratio of NoDGAT2A:2C:2D transcripts, a bank of N. oceanica strains optimized for nutritional supplement or fuel production with a wide range of degree of unsaturation were created, in which proportion of SFAs, MUFAs, and PUFAs in TAG varied by 1.3-, 3.7-, and 11.2-fold, respectively. This established a novel strategy to simultaneously improve productivity and quality of oils from industrial microalgae. |
文章类型 | Article |
关键词 | Biofuels Degree Of Unsaturation Genetic Engineering Diacylglycerol Acyltransferase Nannochloropsis |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1016/j.molp.2017.10.011 |
关键词[WOS] | FATTY-ACID-COMPOSITION ; NANNOCHLOROPSIS-OCEANICA ; TRIACYLGLYCEROL BIOSYNTHESIS ; OLEAGINOUS MICROALGA ; EICOSAPENTAENOIC ACID ; ENDOPLASMIC-RETICULUM ; SYNTHETIC BIOLOGY ; GENE ; IDENTIFICATION ; CHLAMYDOMONAS |
收录类别 | SCI |
语种 | 英语 |
所属项目编号 | (31425002, 31600059, 31571807, 31401116, ZR2015CQ003 |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
项目资助者 | Natural Science Foundation of China(31425002 ; Chinese Academy of Sciences(KSZD-EW-Z-017 ; Natural Science Foundation of Shandong(ZR2015CQ003) ; U.S. National Science Foundation(CBET-1511939) ; U.S. Office of Naval Research(N00014-15-1-2219) ; 31600059 ; ZDRW-ZS-2016-3) ; 31571807 ; 31401116) |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS记录号 | WOS:000417204100005 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9865 |
专题 | 单细胞中心组群 |
通讯作者 | Xu J(徐健) |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA 4.Univ Maryland Baltimore Cty, Baltimore, MD 21202 USA 5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China 6.Peking Univ, Coll Engn, Dept Energy & Resource Engn, Beijing 100871, Peoples R China 7.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China 8.Chinese Acad Sci, Inst Hydrobiol, Diatom Biol Grp, Wuhan 430072, Hubei, Peoples R China 9.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Core Lab, Qingdao 266101, Shandong, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Xin, Yi,Lu, Yandu,Lee, Yi-Ying,et al. Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases[J]. MOLECULAR PLANT,2017,10(12):1523-1539. |
APA | Xin, Yi.,Lu, Yandu.,Lee, Yi-Ying.,Wei, Li.,Jia, Jing.,...&徐健.(2017).Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases.MOLECULAR PLANT,10(12),1523-1539. |
MLA | Xin, Yi,et al."Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases".MOLECULAR PLANT 10.12(2017):1523-1539. |
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