| Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms | |
| Qiang Zhang, Tingting Wang, Qian Zhou, Peng Zhang, Yanhai Gong, Honglei Gou, Jian Xu and Bo Ma; Bo Ma; Jian Xu | |
| 2017-01-23 | |
| 发表期刊 | Scientific reports
 |
| 期号 | 7页码:41192 |
| 摘要 | Wider application of single-cell analysis has been limited by the lack of an easy-to-use and low-cost strategy for single-cell isolation that can be directly coupled to single-cell sequencing and single-cell cultivation, especially for small-size microbes. Herein, a facile droplet microfluidic platform was developed to dispense individual microbial cells into conventional standard containers for downstream analysis. Functional parts for cell encapsulation, droplet inspection and sorting, as well as a chip-to-tube capillary interface were integrated on one single chip with simple architecture, and control of the droplet sorting was achieved by a low-cost solenoid microvalve. Using microalgal and yeast cells as models, single-cell isolation success rate of over 90% and single-cell cultivation success rate of 80% were demonstrated. We further showed that the individual cells isolated can be used in high-quality DNA and RNA analyses at both gene-specific and whole-genome levels (i.e. real-time quantitative PCR and genome sequencing). The simplicity and reliability of the method should improve accessibility of single-cell analysis and facilitate its wider application in microbiology researches. |
| 文章类型 | Article |
| 关键词 | 无 |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1038/srep41192 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392419800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9862 |
| 专题 | 单细胞中心组群 |
| 通讯作者 | Bo Ma; Jian Xu |
| 推荐引用方式 GB/T 7714 | Qiang Zhang, Tingting Wang, Qian Zhou, Peng Zhang, Yanhai Gong, Honglei Gou, Jian Xu and Bo Ma,Bo Ma,Jian Xu. Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms[J]. Scientific reports,2017(7):41192. |
| APA | Qiang Zhang, Tingting Wang, Qian Zhou, Peng Zhang, Yanhai Gong, Honglei Gou, Jian Xu and Bo Ma,Bo Ma,&Jian Xu.(2017).Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms.Scientific reports(7),41192. |
| MLA | Qiang Zhang, Tingting Wang, Qian Zhou, Peng Zhang, Yanhai Gong, Honglei Gou, Jian Xu and Bo Ma,et al."Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms".Scientific reports .7(2017):41192. |
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