| Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells | |
Wang, Xixian1,2,4; Ren, Lihui1,2,4 ; Su, Yetian1,2; Ji, Yuetong1,2,4; Liu, Yaoping5; Li, Chunyu1,2,4; Li, Xunrong1,2,4; Zhang, Yi1,2; Wang, Wei5; Hu, Qiang3; Han, Danxiang3; Xu, Jian1,2,4; Ma, Bo1,2,4
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| 2017-11-21 | |
| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 卷号 | 89期号:22页码:12569-12577 |
| 摘要 | Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated Raman-activated droplet sorting (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as a model. Raman microspectroscopy analysis of individual cells is carried out prior to their microdroplet encapsulation, which is then directly coupled to DEP-based droplet sorting. To validate the system, H. pluvialis cells containing different levels of AXT were mixed and underwent RADS. Those AXT-hyperproducing cells were sorted with an accuracy of 98.3%, an enrichment ratio of eight folds, and a throughput of similar to 260 cells/min. Of the RADS-sorted cells, 92.7% remained alive and able to proliferate, which is equivalent to the unsorted cells. Thus, the RADS achieves a much higher throughput than existing RACS systems, preserves the vitality of cells, and facilitates seamless coupling with downstream manipulations such as single-cell sequencing and cultivation. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1021/acs.analchem.7b03884 |
| 关键词[WOS] | HAEMATOCOCCUS-PLUVIALIS MUTANTS ; MICROFLUIDIC FLOW ; MICROBIAL-CELLS ; STRESS-RESPONSE ; GREEN-ALGA ; SPECTROSCOPY ; ASTAXANTHIN ; EVOLUTION ; TWEEZERS ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| 项目资助者 | China Postdoctoral Science Foundation ; National Natural Science Foundation of China(31600076 ; Ministry of Science and Technology of China(2016YFF0202303) ; Chinese Academy of Sciences(KFJ-SW-STS-165 ; China Postdoctoral Science Foundation(2017M612364 ; Shandong Province Natural Science Foundation(ZR2014CQ005) ; State Development & Investment Corporation (SDIC) ; 31470220 ; XDBP0305) ; 2017T100522) ; 31670101 ; 31327001 ; 31601084 ; 21775155 ; 31425002) |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000416498100092 |
| 出版者 | AMER CHEMICAL SOC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9861 |
| 专题 | 单细胞中心组群 |
| 通讯作者 | Xu, Jian; Ma, Bo |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biofuels & Biotechnol, Wuhan 430072, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xixian,Ren, Lihui,Su, Yetian,et al. Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells[J]. ANALYTICAL CHEMISTRY,2017,89(22):12569-12577. |
| APA | Wang, Xixian.,Ren, Lihui.,Su, Yetian.,Ji, Yuetong.,Liu, Yaoping.,...&Ma, Bo.(2017).Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells.ANALYTICAL CHEMISTRY,89(22),12569-12577. |
| MLA | Wang, Xixian,et al."Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells".ANALYTICAL CHEMISTRY 89.22(2017):12569-12577. |
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