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Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells
Wang, Xixian1,2,4; Ren, Lihui1,2,4; Su, Yetian1,2; Ji, Yuetong1,2,4; Liu, Yaoping5; Li, Chunyu1,2,4; Li, Xunrong1,2,4; Zhang, Yi1,2; Wang, Wei5; Hu, Qiang3; Han, Danxiang3; Xu, Jian1,2,4; Ma, Bo1,2,4
2017-11-21
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume89Issue:22Pages:12569-12577
AbstractRaman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated Raman-activated droplet sorting (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as a model. Raman microspectroscopy analysis of individual cells is carried out prior to their microdroplet encapsulation, which is then directly coupled to DEP-based droplet sorting. To validate the system, H. pluvialis cells containing different levels of AXT were mixed and underwent RADS. Those AXT-hyperproducing cells were sorted with an accuracy of 98.3%, an enrichment ratio of eight folds, and a throughput of similar to 260 cells/min. Of the RADS-sorted cells, 92.7% remained alive and able to proliferate, which is equivalent to the unsorted cells. Thus, the RADS achieves a much higher throughput than existing RACS systems, preserves the vitality of cells, and facilitates seamless coupling with downstream manipulations such as single-cell sequencing and cultivation.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1021/acs.analchem.7b03884
WOS KeywordHAEMATOCOCCUS-PLUVIALIS MUTANTS ; MICROFLUIDIC FLOW ; MICROBIAL-CELLS ; STRESS-RESPONSE ; GREEN-ALGA ; SPECTROSCOPY ; ASTAXANTHIN ; EVOLUTION ; TWEEZERS ; IDENTIFICATION
Indexed BySCI
Language英语
WOS Research AreaChemistry
Funding OrganizationChina Postdoctoral Science Foundation ; National Natural Science Foundation of China(31600076 ; Ministry of Science and Technology of China(2016YFF0202303) ; Chinese Academy of Sciences(KFJ-SW-STS-165 ; China Postdoctoral Science Foundation(2017M612364 ; Shandong Province Natural Science Foundation(ZR2014CQ005) ; State Development & Investment Corporation (SDIC) ; 31470220 ; XDBP0305) ; 2017T100522) ; 31670101 ; 31327001 ; 31601084 ; 21775155 ; 31425002)
WOS SubjectChemistry, Analytical
WOS IDWOS:000416498100092
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9861
Collection单细胞中心组群
Corresponding AuthorXu, Jian; Ma, Bo
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biofuels & Biotechnol, Wuhan 430072, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xixian,Ren, Lihui,Su, Yetian,et al. Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells[J]. ANALYTICAL CHEMISTRY,2017,89(22):12569-12577.
APA Wang, Xixian.,Ren, Lihui.,Su, Yetian.,Ji, Yuetong.,Liu, Yaoping.,...&Ma, Bo.(2017).Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells.ANALYTICAL CHEMISTRY,89(22),12569-12577.
MLA Wang, Xixian,et al."Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells".ANALYTICAL CHEMISTRY 89.22(2017):12569-12577.
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