脂肪酶—P450脂肪酸脱羧酶融合蛋白催化的α-烯烃合成

doi:doi: 10.1360/N972017-00212

QIBEBT-IR > 酶工程研究组

	脂肪酶—P450脂肪酸脱羧酶融合蛋白催化的α-烯烃合成
	孙月; 马莉; 陈辉; 徐卉芳; 郑贤良; 齐凤霞; 李盛英
	2017
发表期刊	科学通报
卷号	62 期号:1 页码:1-7
其他摘要	The irreversibly diminishing reserves and the fluctuating price of crude oil, together with the serious concerns on the global environment and energy security, all suggests an unpleasant future of fossil fuel. Within this context, it is imperative to find alternative eco-friendly fuel with properties that highly mimic fossil fuel. Aliphatic alka(e)nes generated from renewable resources via biosynthetic pathways perfectly suits the criteria and represents the ideal “drop-in” biofuels. α-Alkenes are special microbial natural products. These biohydrocarbons have attracted much attention due to their potential application as biofuels and biomaterials. By coupling the activity of lipase and P450 decarboxylase, lipids (i.e. triglycerides) can be transformed into α-alkenes via free fatty acid intermediates. To improve the conversion efficiency of lipids to α-alkenes, in this study, four different versions of fusion proteins of the lipase TlL from Thermomyces lanuginosus and the P450 decarboxylase OleTJE from Jeotgalicoccus sp. ATCC 8456, including FusA (His6-TlL-linker-OleTJE), FusB (TlL-Linker-OleTJE-His6), FusC (His6-OleTJE-linker-TlL) and FusD (OleTJE- linker-TlL-His6), were constructed and comparatively evaluated. The optimal fusion enzyme FusC was able to convert a number of triacylglycerols including trilaurin (C12), trimyristin (C14) and tripalmitin (C16) into corresponding α-alkenes via the coupled hydrolysis and decarboxylation. The 31.7% yield of 1-tridecene from trimyristin demonstrates good substrate channeling effect. Due to the higher catalytic efficiency compared to the mixed TlL and OleTJE, as well as the simplified enzyme purification and hence the lower cost, this fusion strategy may hold significant potential of application.
文章类型	Article
DOI	doi: 10.1360/N972017-00212
关键词[WOS]	脂肪酶 ; P450 脂肪酸脱羧酶 ; 融合蛋白 ; α烯烃 ; 底物通道效应
语种	中文
WOS研究方向	Microbiology
项目资助者	国家自然科学基金(31422002, 21406250, 31270855) ; 山东省自然科学杰出青年基金(JQ201407) ; 青岛市应用基础研究计划(15-9-1-106-jch)
WOS类目	Microbiology
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9859
专题	酶工程研究组
推荐引用方式 GB/T 7714	孙月,马莉,陈辉,等. 脂肪酶—P450脂肪酸脱羧酶融合蛋白催化的α-烯烃合成[J]. 科学通报,2017,62(1):1-7.
APA	孙月.,马莉.,陈辉.,徐卉芳.,郑贤良.,...&李盛英.(2017).脂肪酶—P450脂肪酸脱羧酶融合蛋白催化的α-烯烃合成.科学通报,62(1),1-7.
MLA	孙月,et al."脂肪酶—P450脂肪酸脱羧酶融合蛋白催化的α-烯烃合成".科学通报 62.1(2017):1-7.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
脂肪酶—P450脂肪酸脱羧酶融合蛋白催化（1006KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	浏览下载