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Title:
DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation
Author: Zongchang Xu , Meng Wang, Dachuan Shi , Gongke Zhou, Tiantian Niu, Michael G. Hahn, Malcolm A. O’Neill , Yingzhen Kong
Source: Plant Science
Issued Date: 2017-01-30
Volume: 258, Pages:156-169
DOI: DOI:10.1016/j.plantsci.2017.01.005
DOC Type: Article
English Abstract: Our previous study of the Arabidopsis mur3-3 mutant and mutant plants in which the mur3-3 phenotypes are suppressed (xxt2mur3-3, xxt5mur3-3, xxt1xxt2mur3-3 and 35Spro:XLT2:mur3-3) showed that hypocotyl cell elongation is decreased in plants that synthesize galactose-deficient xyloglucan. To obtain genome-wide insight into the transcriptome changes and regulatory networks that may be involved in this decreased elongation, we performed digital gene expression analyses of the etiolated hypocotyls of wild type (WT), mur3-3 and the four suppressor lines. Numerous differentially expressed genes (DEGs) were detected in comparisons between WT and mur3-3 (1423), xxt2mur3-3 and mur3-3 (675), xxt5mur3-3 and mur3-3 (1272), xxt1xxt2mur3-3 and mur3-3 (1197) and 35Spro:XLT2:mur3-3 vs mur3-3 (121). 550 overlapped DEGs were detected among WT vs mur3-3, xxt2mur3-3 vs mur3-3, xxt5mur3-3 vs mur3-3, and xxt1xxt2mur3-3 vs mur3-3 comparisons. These DEGs include 46 cell wall-related genes, 24 transcription factors, 6 hormone-related genes, 9 protein kinase genes and 9 aquaporin genes. The expression of all of the 550 overlapped genes is restored to near wild-type levels in the four mur3-3 suppressor lines. qRT-PCR of fifteen of these 550 genes showed that their expression levels are consistent with the digital gene expression data. Overexpression of some of these genes (XTH4, XTH30, PME3, EXPA11, MYB88, ROT3, AT5G37790, WAG2 and TIP2;3) that are down-regulated in mur3-3 partially rescued the short hypocotyl phenotype but not the aerial phenotype of mur3-3, indicating that different mechanisms exist between hypocotyl cell elongation and leaf cell elongation. (C) 2017 Elsevier B.V. All rights reserved
WOS Keyword Plus: Arabidopsis ; MUR3 ; Xyloglucan ; Cell elongation ; Hypocotyl ; DGE-seq
Indexed Type: SCI
Funder: the Taishan Scholar Program of Shandong (to GZ) and The Division of Chemical Sciences, Geosciences, and Bio- sciences,
Language: 英语
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/9832
Appears in Collections:植物代谢工程团队_期刊论文

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Recommended Citation:
Zongchang Xu , Meng Wang, Dachuan Shi , Gongke Zhou, Tiantian Niu, Michael G. Hahn, Malcolm A. O’Neill , Yingzhen Kong. DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation[J]. Plant Science,2017,258:156-169.
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