A Post Processing Method for Optimizing Synthesis Strategy for Oligonucleotide Microarrays | |
Kang Ning; Kwok Pui Choi; Hon Wai Leong; Louxin Zhang | |
2005 | |
发表期刊 | Nucleic Acids Research |
期号 | 11 |
摘要 | The broad applicability of gene expression profiling to genomic analyses has generated huge demand for mass production of microarrays and hence for improving the cost effectiveness of microarray fabrication. We developed a post-processing method for deriving a good synthesis strategy. In this paper, we assessed all the known efficient methods and our post-processing method for reducing the number of synthesis cycles for manufacturing a DNA-chip of a given set of oligos. Our experimental results on both simulated and 52 real datasets show that no single method consistently gives the best synthesis strategy, and post-processing an existing strategy is necessary as it often reduces the number of synthesis cycles further. ; The broad applicability of gene expression profiling to genomic analyses has generated huge demand formass production of microarrays and hence for improving the cost effectiveness of microarray fabrication. We developed a post-processing method for deriving a good synthesis strategy. In this paper, we assessed all the known efficient methods and our post-processing method for reducing the number of synthesis cycles for manufacturing a DNA-chip of a given set of oligos. Our experimental results on both simulated and 52 real datasets show that no single method consistently gives the best synthesis strategy, and post-processing an existing strategy is necessary as it often reduces the number of synthesis cycles further. |
关键词 | a Post Processing Method For Optimizing Synthesis Strategy For Oligonucleotide Microarrays |
学科领域 | 功能基因组 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/980 |
专题 | 单细胞中心组群 |
推荐引用方式 GB/T 7714 | Kang Ning,Kwok Pui Choi,Hon Wai Leong,et al. A Post Processing Method for Optimizing Synthesis Strategy for Oligonucleotide Microarrays[J]. Nucleic Acids Research,2005(11). |
APA | Kang Ning,Kwok Pui Choi,Hon Wai Leong,&Louxin Zhang.(2005).A Post Processing Method for Optimizing Synthesis Strategy for Oligonucleotide Microarrays.Nucleic Acids Research(11). |
MLA | Kang Ning,et al."A Post Processing Method for Optimizing Synthesis Strategy for Oligonucleotide Microarrays".Nucleic Acids Research .11(2005). |
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