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Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth
Ge, Anle1; Hu, Liang1; Wang, Xixian1,2,3; Zhu, Jinchi1; Feng, Xiaojun1; Du, Wei1; Liu, Bi-Feng1
2018-02-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号255页码:735-744
摘要C. elegans is a commonly used model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL(-1) and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen. (C) 2017 Elsevier B.V. All rights reserved.
文章类型Article
关键词Microfluidic Device Caenorhabditis Elegans Logarithmic Bacterial Gradient Dietary Restriction Lifespan Extension
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1016/j.snb.2017.08.088
关键词[WOS]CAENORHABDITIS-ELEGANS ; MICROFLUIDIC DEVICE ; QUANTITATIVE-ANALYSIS ; DEVELOPMENTAL-STAGES ; LIFE-SPAN ; GENETICS ; PLATFORM ; CHEMOTAXIS ; LONGEVITY ; PATHWAYS
收录类别SCI
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者National Natural Science Foundation of China(21475049 ; National Key R&D Program of China(2016YFF0100801) ; NIH National Centre for Research Resources ; 31471257 ; 21275060)
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000414151800088
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9737
专题单细胞中心组群
作者单位1.Huazhong Univ Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Key Lab Biomed Photon,Syst Biol Theme,Dept Biomed, MOE,Wuhan Natl Lab Optoelect,Coll Life Sci & Tech, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
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Ge, Anle,Hu, Liang,Wang, Xixian,et al. Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:735-744.
APA Ge, Anle.,Hu, Liang.,Wang, Xixian.,Zhu, Jinchi.,Feng, Xiaojun.,...&Liu, Bi-Feng.(2018).Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth.SENSORS AND ACTUATORS B-CHEMICAL,255,735-744.
MLA Ge, Anle,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):735-744.
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