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Entropy drives the formation of salt bridges in the protein GB3
Ning Zhang,; Yefei Wang,; Liaoyuan An; Xiangfei Song; Qingshan Huang,; Zhijun Liu; Lishan Yao
2017-06-19
发表期刊Angewandte Chemie International Edition
卷号56期号:26页码:7601-7604
产权排序(1)Shandong Provincial Key Laboratory of Synthetic Biology Qingdao Institute of Bioenergy and Bioprocess Technology University of Chinese Academy of Sciences;(2)Department National Center for Protein Science Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
摘要Salt bridges are very common in proteins. But what drives the formation of protein salt bridges is not clear. In this work, we determined the strength of four salt bridges in the protein GB3 by measuring the DpKa values of the basic residues that constitute the salt bridges with a highly accurate NMR titration method at different temperatures. The results show that the DpKa values increase with temperature, thus indicating that the salt bridges are stronger at higher temperatures. Fitting of DpKa values to the vanQt Hoff equation yields positive DH and DS values, thus indicating that entropy drives salt-bridge formation. Molecular dynamics simulations show that the protein and solvent make opposite contributions to DH and DS. Specifically, the enthalpic gain contributed from the protein is more than offset by the enthalpic loss contributed from the solvent, whereas the entropic gain originates from the desolvation effect.
文章类型Article
关键词Enthalpy Entropy Nmr Spectroscopy Protein Structures Salt Bridges
WOS标题词Science & Technology ; Physical Sciences
DOI10.1002/anie.201702968
关键词[WOS]enthalpy ;  entropy ;  NMR spectroscopy ;  protein structures ;  salt bridges
收录类别SCI
语种英语
所属项目编号31661143036  ; 31600051  ; 21376254 ; 31600690
WOS研究方向Chemistry, Multidisciplinary
项目资助者National Natural Science Foundation of China
WOS类目Chemistry
WOS记录号WOS:000403017000048
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9710
专题仿真与模拟团队
通讯作者Lishan Yao
作者单位1.Shandong Provincial Key Laboratory of Synthetic Biology Qingdao Institute of Bioenergy and Bioprocess Technology University of Chinese Academy of Sciences
2.Department National Center for Protein Science Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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Ning Zhang,,Yefei Wang,,Liaoyuan An,et al. Entropy drives the formation of salt bridges in the protein GB3[J]. Angewandte Chemie International Edition,2017,56(26):7601-7604.
APA Ning Zhang,.,Yefei Wang,.,Liaoyuan An.,Xiangfei Song.,Qingshan Huang,.,...&Lishan Yao.(2017).Entropy drives the formation of salt bridges in the protein GB3.Angewandte Chemie International Edition,56(26),7601-7604.
MLA Ning Zhang,,et al."Entropy drives the formation of salt bridges in the protein GB3".Angewandte Chemie International Edition 56.26(2017):7601-7604.
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