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Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
Zhang, Jie1,2,3,4; Liu, Shiyue1,2,3,4; Li, Renmin1,2,3,4; Hong, Wei5; Xiao, Yan1,2,3; Feng, Yingang1,2,3; Cui, Qiu1,2,3; Liu, Ya-Jun1,2,3
2017-05-12
发表期刊BIOTECHNOLOGY FOR BIOFUELS
卷号10
摘要Background: Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost catalyst for lignocellulose saccharification. The industrial use of C. thermocellum is restrained due largely to the inhibition of the hydrolysate cellobiose to its cellulosome. Although the supplementation of beta-glucosidase may solve the problem, the production of the enzymes greatly complicates the process and may also increase the cost of saccharification.
文章类型Article
关键词Beta-glucosidase Cellulosome Cels Fermentable Sugar Genome Editing Lignocellulose
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1186/s13068-017-0796-y
关键词[WOS]TARGETED GENE DELETION ; BETA-GLUCOSIDASE ; THERMOANAEROBACTER-BROCKII ; DEGRADATION ; PROTEIN ; MICROBES ; ENZYME ; POLYSACCHARIDES ; SOLUBILIZATION ; INTEGRATION
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者National Natural Science Foundation of China(31300035 ; National Key Technology Research and Development Program of China(2015BAD15B05) ; Shandong Province Key Laboratory Union of Carbohydrate Industry Science and Technology ; 31470210 ; 31560318 ; 31570029 ; 31570787)
WOS类目Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号WOS:000401620700001
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9705
专题代谢物组学研究组
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China
3.Chinese Acad Sci, Qingdao Engn Lab Single Cell Oil, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
4.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing 100049, Peoples R China
5.Guizhou Med Univ, Minist Educ, Key Lab Endem & Ethn Dis, Guiyang 550000, Peoples R China
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Zhang, Jie,Liu, Shiyue,Li, Renmin,et al. Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum[J]. BIOTECHNOLOGY FOR BIOFUELS,2017,10.
APA Zhang, Jie.,Liu, Shiyue.,Li, Renmin.,Hong, Wei.,Xiao, Yan.,...&Liu, Ya-Jun.(2017).Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum.BIOTECHNOLOGY FOR BIOFUELS,10.
MLA Zhang, Jie,et al."Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum".BIOTECHNOLOGY FOR BIOFUELS 10(2017).
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