Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells

doi:10.1186/s13068-017-0967-x

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	Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells
	He, Yuehui 1,2,3; Zhang, Peng 1,2,3; Huang, Shi 1,2,3; Wang, Tingting 1,2,3; Ji, Yuetong 1,2,3; Xu, Jian 1,2,3,4
	2017-11-17
发表期刊	BIOTECHNOLOGY FOR BIOFUELS
卷号	10
摘要	Background: Current approaches for quantification of major energy-storage forms in microalgae, including starch, protein and lipids, generally require cell cultivation to collect biomass followed by tedious and time-consuming analytical procedures. Thus, label-free, non-destructive and simultaneous quantification of such macromolecules at single-cell resolution is highly desirable in microalgal feedstock development and bioprocess control.
文章类型	Article
关键词	Single-cell Raman Spectroscopy Starch Content Protein Content Triacylglycerol Content Phenotypic Heterogeneity Sampling Depth Chlamydomonas Reinhardtii Microalgae
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1186/s13068-017-0967-x
关键词[WOS]	RAMAN-SPECTROSCOPY ; MICROBIAL-CELLS ; YEAST-CELLS ; HETEROGENEITY ; MICROSPECTROSCOPY ; LIVE ; POPULATIONS ; METABOLITES ; ROBUSTNESS ; MECHANISMS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者	NSFC(31327001 ; CAS(XDB15040100) ; China Postdoctoral Science Foundation Grant(2016LH00036) ; Shandong Province(ZR2014CQ005 ; State Development & Investment Corporation (SDIC) ; 31425002 ; 2015ZDJS04002) ; 31400089)
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000415578800001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9696
专题	单细胞中心组群
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	He, Yuehui,Zhang, Peng,Huang, Shi,et al. Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells[J]. BIOTECHNOLOGY FOR BIOFUELS,2017,10.
APA	He, Yuehui,Zhang, Peng,Huang, Shi,Wang, Tingting,Ji, Yuetong,&Xu, Jian.(2017).Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells.BIOTECHNOLOGY FOR BIOFUELS,10.
MLA	He, Yuehui,et al."Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells".BIOTECHNOLOGY FOR BIOFUELS 10(2017).