Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation

doi:10.1016/j.chemosphere.2017.09.146

QIBEBT-IR > 资源植物与环境工程研究组

	Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation
	Xu, Hua1 ; Yu, Changjiang1,2 ; Xia, Xinli 3; Li, Mingliang 1; Li, Huiguang 3; Wang, Yu1 ; Wang, Shumin1 ; Wang, Congpeng 1; Ma, Yubin1 ; Zhou, Gongke 1
	2018
发表期刊	CHEMOSPHERE
卷号	190 页码:154-165
摘要	Cadmium (Cd) is a detrimental environmental pollutant. Duckweeds have been considered promising candidates for Cd phytoremediation. Although many physiological studies have been conducted, the molecular mechanisms underlying Cd hyperaccumulation in duckweeds are largely unknown. In this study, clone 6001 of Landoltia punctata, which showed high Cd tolerance, was obtained by large-scale screening of over 200 duckweed clones. Subsequently, its growth, Cd flux, Cd accumulation, and Cd distribution characteristics were investigated. To further explore the global molecular mechanism, a comprehensive transcriptome analysis was performed. For RNA-Seq, samples were treated with 20 mu M CdCl2 for 0,1, 3, and 6 days. In total, 9,461, 9,847, and 9615 differentially expressed unigenes (DEGs) were discovered between Cd-treated and control (0 day) samples. DEG clustering and enrichment analysis identified several biological processes for coping with Cd stress. Genes involved in DNA repair acted as an early response to Cd, while RNA and protein metabolism would be likely to respond as well. Furthermore, the carbohydrate metabolic flux tended to be modulated in response to Cd stress, and upregulated genes involved in sulfur and ROS metabolism might cause high Cd tolerance. Vacuolar sequestration most likely played an important role in Cd detoxification in L punctata 6001. These novel findings provided important clues for molecular assisted screening and breeding of Cd hyperaccumulating cultivars for phytoremediation. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Cadmium Duckweed Transcriptome Detoxification Sequestration Phytoremediation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.chemosphere.2017.09.146
关键词[WOS]	LEMNA-MINOR ; PHYSIOLOGICAL-RESPONSES ; ENVIRONMENTAL-STRESS ; GENE-EXPRESSION ; MISMATCH REPAIR ; PLANT-GROWTH ; HEAVY-METALS ; WASTE-WATER ; DNA-REPAIR ; POPULUS X
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology
项目资助者	Natural Science Foundation of China (NSFC)(51509237 ; National Key Technology Research and Development Program of China(2015BAD15B01) ; Natural Science Foundation of Shandong Province(ZR2015PE012) ; Key Project of Research and Development Plan of Shandong Province(2016GNC113020) ; Taishan Scholar Program of Shandong ; U1632140)
WOS类目	Environmental Sciences
WOS记录号	WOS:000414881600017
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9686
专题	资源植物与环境工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels,Shandong Prov Key Lab Energy Gen, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Natl Engn Lab Tree Breeding, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Xu, Hua,Yu, Changjiang,Xia, Xinli,et al. Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation[J]. CHEMOSPHERE,2018,190:154-165.
APA	Xu, Hua.,Yu, Changjiang.,Xia, Xinli.,Li, Mingliang.,Li, Huiguang.,...&Zhou, Gongke.(2018).Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation.CHEMOSPHERE,190,154-165.
MLA	Xu, Hua,et al."Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation".CHEMOSPHERE 190(2018):154-165.