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Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis
Huang, Shi1; Yang, Fang4,5; Zeng, Xiaowei1; Chen, Jie1; Li, Rui2; Wen, Ting2; Li, Chun2; Wei, Wei2; Liu, Jiquan2; Chen, Lan2; Davis, Catherine3; Xu, Jian1
2011
Source PublicationBMC ORAL HEALTH
Volume11Issue:11
Abstract

Background: Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing. Methods: Six non-smoking Chinese, three with and three without gingivitis (age range 21-39 years, 4 females and 2 males) were enrolled in the present cross-sectional study. Gingival parameters of inflammation and bleeding on probing were characterized by a clinician using the Mazza Gingival Index (MGI). Plaque (sampled separately from four different oral sites) and salivary samples were obtained from each subject. Sequences and relative abundance of the bacterial 16 S rDNA PCR-amplicons were determined via pyrosequencing that produced 400 bp-long reads. The sequence data were analyzed via a computational pipeline customized for human oral microbiome analyses. Furthermore, the relative abundances of selected microbial groups were validated using quantitative PCR. Results: The oral microbiomes from gingivitis and healthy subjects could be distinguished based on the distinct community structures of plaque microbiomes, but not the salivary microbiomes. Contributions of community members to community structure divergence were statistically accessed at the phylum, genus and species-like levels. Eight predominant taxa were found associated with gingivitis: TM7, Leptotrichia, Selenomonas, Streptococcus, Veillonella, Prevotella, Lautropia, and Haemophilus. Furthermore, 98 species-level OTUs were identified to be gingivitis-associated, which provided microbial features of gingivitis at a species resolution. Finally, for the two selected genera Streptococcus and Fusobacterium, Real-Time PCR based quantification of relative bacterial abundance validated the pyrosequencing-based results. Conclusions: This methods study suggests that oral samples from this patient population of gingivitis can be characterized via plaque microbiome by pyrosequencing the 16 S rDNA genes. Further studies that characterize serial samples from subjects (longitudinal study design) with a larger population size may provide insight into the temporal and ecological features of oral microbial communities in clinically-defined states of gingivitis.

; Background: Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing.
SubtypeArticle
KeywordOral Microbiota Gingivitis Saliva Plaque Pyrosequencing
Subject Area功能基因组
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1186/1472-6831-11-33
WOS KeywordHUMAN SUBGINGIVAL CREVICE ; BACTERIAL DIVERSITY ; MOLECULAR ANALYSIS ; RARE BIOSPHERE ; CARIOUS DENTIN ; COMMUNITIES ; TM7 ; IDENTIFICATION ; MICROFLORA ; CHILDREN
Indexed BySCI
Language英语
WOS Research AreaDentistry, Oral Surgery & Medicine
WOS SubjectDentistry, Oral Surgery & Medicine
WOS IDWOS:000208596800033
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/966
Collection单细胞中心组群
Affiliation1.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China
2.Procter & Gamble Innovat Ctr, Beijing 101312, Peoples R China
3.Procter & Gamble Winton Hill Business Ctr, Cincinnati, OH 45224 USA
4.Qingdao Municipal Hosp, Dept Stomatol, Qingdao 266011, Shandong, Peoples R China
5.Sun Yat Sen Univ, Guanghua Sch & Hosp Stomatol, Dept Operat Dent & Endodont, Guangzhou 510055, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Huang, Shi,Yang, Fang,Zeng, Xiaowei,et al. Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis[J]. BMC ORAL HEALTH,2011,11(11).
APA Huang, Shi.,Yang, Fang.,Zeng, Xiaowei.,Chen, Jie.,Li, Rui.,...&Xu, Jian.(2011).Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis.BMC ORAL HEALTH,11(11).
MLA Huang, Shi,et al."Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis".BMC ORAL HEALTH 11.11(2011).
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