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Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application
Zhao, Fuhua1,2; Wang, Qiao1,2; Dong, Jianjun1; Xian, Mo2; Yu, Junhong1; Yin, Hua1; Chang, Zongming1; Mu, Xindong2; Hou, Tonggang2; Wang, Jianxun2
2017-06-01
发表期刊PROCESS BIOCHEMISTRY
卷号57页码:87-94
摘要In this work, novel nanoflower/alginate microbeads were synthesized to immobilize alpha-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca-3(PO4)(2) to form enzyme inorganic hybrid nanoflowers (Ca-3(PO4)(2)-ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca-3(PO4)(2)-ALDC)@Mg). Compared with free ALDC and Ca-3(PO4)(2)-ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300 L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry.
文章类型Article
关键词Enzyme-inorganic Nanoflowers Microbeads Alginate Enzyme Immobilization Diacetyl
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2017.03.026
关键词[WOS]ALPHA-ACETOLACTATE DECARBOXYLASE ; CA-ALGINATE BEADS ; HYBRID NANOFLOWERS ; GRAPHENE OXIDE ; EFFICIENT METHOD ; ENCAPSULATION ; LIPASE ; MICROCAPSULES ; ENTRAPMENT ; CAPSULES
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
项目资助者National Natural Science Foundation of China(21433001 ; Applied Basic Research Foundation of Qingdao City(14-2-4-21-jch) ; 21603251)
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号WOS:000403121600012
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9650
专题生物基材料组群
作者单位1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266100, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China
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Zhao, Fuhua,Wang, Qiao,Dong, Jianjun,et al. Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application[J]. PROCESS BIOCHEMISTRY,2017,57:87-94.
APA Zhao, Fuhua.,Wang, Qiao.,Dong, Jianjun.,Xian, Mo.,Yu, Junhong.,...&Wang, Jianxun.(2017).Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application.PROCESS BIOCHEMISTRY,57,87-94.
MLA Zhao, Fuhua,et al."Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application".PROCESS BIOCHEMISTRY 57(2017):87-94.
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