The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803

doi:10.3389/fmicb.2017.01176

QIBEBT-IR > 微生物代谢工程研究组

	The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803
	Song, Kuo 1; Hagemann, Martin 2; Tan, Xiaoming 1; Lu, Xuefeng 1,3
	2017-06-26
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	8
摘要	Cyanobacterial sucrose biosynthesis is stimulated under salt stress, which could be used for biotechnological sugar production. It has been shown that the response regulator Slr1588 negatively regulates the spsA gene encoding sucrose-phosphate synthase and mutation of the slr1588 gene also affected the salt tolerance of Synechocystis (Chen et al., 2014). The latter finding is contrary to earlier observations (Hagemann et al., 1997b). Here, we observed that ectopic expression of slr1588 did not restore the salt tolerance of the slr1588 mutant, making the essential function of this response regulator for salt tolerance questionable. Subsequent experiments showed that deletion of the entire coding sequence of slr1588 compromised the expression of the downstream situated ggpP gene, which encodes glucosylglycerol-phosphate phosphatase for synthesis of the primary osmolyte glucosylglycerol. Mutation of Delta slr1588 by deleting the N-terminal part of this protein (Delta slr1588-F976) did not affect ggpP expression, glucosylglycerol accumulation as well as salt tolerance, while the mutation of ggpP resulted in the previously reported salt-sensitive phenotype. In the Delta slr1588-F976 mutant spsA was up-regulated but sucrose content was lowered due to increased invertase activity. Our results reveal that Slr1588 is acting as a repressor for spsA as previously suggested but it is not crucial for the overall salt acclimation of Synechocystis.
文章类型	Article
关键词	Slr1588 Synechocystis Spsa Glucosylglycerol Sucrose Ggpp Response Regulator Transcriptional Regulation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fmicb.2017.01176
关键词[WOS]	SP PCC-6803 ; PHOSPHATE-SYNTHASE ; COMPATIBLE SOLUTE ; CYANOBACTERIA ; GENE ; SUCROSE ; GLUCOSYLGLYCEROL ; EXPRESSION ; ENCODES ; ACCUMULATION
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	National Science Fund for Distinguished Young Scholars of China(31525002) ; Joint Sino-German Research Project(GZ 984) ; Shandong Taishan Scholarship ; National Science Foundation of China(31301018) ; Key Research Program of the Chinese Academy of Sciences(ZDRW-ZS-2016-3)
WOS类目	Microbiology
WOS记录号	WOS:000404006100002
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9644
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Univ Rostock, Inst Biol Sci, Dept Plant Physiol, Rostock, Germany 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Song, Kuo,Hagemann, Martin,Tan, Xiaoming,et al. The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA	Song, Kuo,Hagemann, Martin,Tan, Xiaoming,&Lu, Xuefeng.(2017).The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803.FRONTIERS IN MICROBIOLOGY,8.
MLA	Song, Kuo,et al."The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803".FRONTIERS IN MICROBIOLOGY 8(2017).