QIBEBT-IR  > 单细胞中心
Single cell Raman spectroscopy for cell sorting and imaging
Li, Mengqiu1; Xu, Jian2,3; Romero-Gonzalez, Maria1; Banwart, Steve A.1; Huang, Wei E.1
2012-02-01
发表期刊CURRENT OPINION IN BIOTECHNOLOGY
卷号23期号:1页码:56-63
摘要

Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular ‘fingerprint’ of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SRS) and surface enhanced Raman scattering (SERS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.

; Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular 'fingerprint' of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SIRS) and surface enhanced Raman scattering (SEAS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.
文章类型Review
学科领域功能基因组
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.copbio.2011.11.019
关键词[WOS]IN-SITU HYBRIDIZATION ; FLUORESCENCE MICROSCOPY ; BACTERIAL-CELLS ; MICROBIAL-CELLS ; BLOOD-CELLS ; SCATTERING ; MICROSPECTROSCOPY ; COHERENT ; MOLECULES ; CYTOMETRY
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目Biochemical Research Methods ; Biotechnology & Applied Microbiology
WOS记录号WOS:000300868100010
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/962
专题单细胞中心
作者单位1.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,et al. Single cell Raman spectroscopy for cell sorting and imaging[J]. CURRENT OPINION IN BIOTECHNOLOGY,2012,23(1):56-63.
APA Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,Banwart, Steve A.,&Huang, Wei E..(2012).Single cell Raman spectroscopy for cell sorting and imaging.CURRENT OPINION IN BIOTECHNOLOGY,23(1),56-63.
MLA Li, Mengqiu,et al."Single cell Raman spectroscopy for cell sorting and imaging".CURRENT OPINION IN BIOTECHNOLOGY 23.1(2012):56-63.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Single cell Raman sp(513KB) 开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Mengqiu]的文章
[Xu, Jian]的文章
[Romero-Gonzalez, Maria]的文章
百度学术
百度学术中相似的文章
[Li, Mengqiu]的文章
[Xu, Jian]的文章
[Romero-Gonzalez, Maria]的文章
必应学术
必应学术中相似的文章
[Li, Mengqiu]的文章
[Xu, Jian]的文章
[Romero-Gonzalez, Maria]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Single cell Raman spectroscopy for cell sorting and imaging.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。