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Title:
Single cell Raman spectroscopy for cell sorting and imaging
Author: Li, Mengqiu1; Xu Jian(徐健)2,3; Romero-Gonzalez, Maria1; Banwart, Steve A.1; Huang, Wei E.1
Source: CURRENT OPINION IN BIOTECHNOLOGY
Issued Date: 2012-02-01
Volume: 23, Issue:1, Pages:56-63
Subject: 生物资源::功能基因组
DOI: 10.1016/j.copbio.2011.11.019
DOC Type: Review
Abstract:

Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular ‘fingerprint’ of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SRS) and surface enhanced Raman scattering (SERS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.

English Abstract: Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular 'fingerprint' of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SIRS) and surface enhanced Raman scattering (SEAS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biochemical Research Methods ; Biotechnology & Applied Microbiology
WOS Subject Extended: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Keyword Plus: IN-SITU HYBRIDIZATION ; FLUORESCENCE MICROSCOPY ; BACTERIAL-CELLS ; MICROBIAL-CELLS ; BLOOD-CELLS ; SCATTERING ; MICROSPECTROSCOPY ; COHERENT ; MOLECULES ; CYTOMETRY
Indexed Type: SCI
Language: 英语
WOS ID: WOS:000300868100010
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/962
Appears in Collections:单细胞中心_期刊论文

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description.institution: 1.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China

Recommended Citation:
Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,et al. Single cell Raman spectroscopy for cell sorting and imaging[J]. CURRENT OPINION IN BIOTECHNOLOGY,2012,23(1):56-63.
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