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Single cell Raman spectroscopy for cell sorting and imaging
Li, Mengqiu1; Xu, Jian2,3; Romero-Gonzalez, Maria1; Banwart, Steve A.1; Huang, Wei E.1
2012-02-01
Source PublicationCURRENT OPINION IN BIOTECHNOLOGY
Volume23Issue:1Pages:56-63
Abstract

Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular ‘fingerprint’ of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SRS) and surface enhanced Raman scattering (SERS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.

; Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular 'fingerprint' of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SIRS) and surface enhanced Raman scattering (SEAS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping.
SubtypeReview
Subject Area功能基因组
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.copbio.2011.11.019
WOS KeywordIN-SITU HYBRIDIZATION ; FLUORESCENCE MICROSCOPY ; BACTERIAL-CELLS ; MICROBIAL-CELLS ; BLOOD-CELLS ; SCATTERING ; MICROSPECTROSCOPY ; COHERENT ; MOLECULES ; CYTOMETRY
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS IDWOS:000300868100010
Citation statistics
Cited Times:87[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/962
Collection单细胞中心
Affiliation1.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,et al. Single cell Raman spectroscopy for cell sorting and imaging[J]. CURRENT OPINION IN BIOTECHNOLOGY,2012,23(1):56-63.
APA Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,Banwart, Steve A.,&Huang, Wei E..(2012).Single cell Raman spectroscopy for cell sorting and imaging.CURRENT OPINION IN BIOTECHNOLOGY,23(1),56-63.
MLA Li, Mengqiu,et al."Single cell Raman spectroscopy for cell sorting and imaging".CURRENT OPINION IN BIOTECHNOLOGY 23.1(2012):56-63.
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