Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801

doi:10.1021/acs.jafc7b01533

QIBEBT-IR > 分子微生物工程研究组

	Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801
	Liang, Yunxiao 1; Ma, Xiaoqing2 ; Zhang, Lujia 1,3; Li, Fuli2 ; Liu, Zhen1 ; Mao, Xiangzhao 1
	2017-09-13
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号	65 期号:36 页码:7982-7988
摘要	Agarases are important hydrolytic enzymes for the biodegradation of agar. Understanding the degradation mode and hydrolysis products of agarases is essential for their utilization in oligosaccharide preparations. Herein, we cloned and expressed AgW1150B, a novel neoagarotetraose-forming beta-agarase from Agarivorans gilvus WHO801 that has high specific activity and a fast reaction rate. AgW1150B consists of a C-terminal glycoside hydrolase family 50 catalytic domain with two tandem noncatalytic carbohydrate-binding modules (CBMs) in the N-terminus (residues 45-214 and 236-442). AgWHSOB exhibited good enzymatic properties with high specific activity and catalytic efficiency (1523.2 U/mg and a V-max of 1700 mu mol/min/mg) under optimal hydrolysis conditions of pH 7.0 and 40 degrees C. Analysis of the hydrolysis products revealed that this enzyme is an exotype beta-agarase and that the dominant product of agarose or oligosaccharide degradation was neoagarotetraose. These findings suggest that AgVVH5OB could be utilized to yield abundant neoagarotetraose.
文章类型	Article
关键词	Agarase Ghso Family Agarivorans Gilvus Degradation Mode
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1021/acs.jafc7b01533
关键词[WOS]	MARINE BACTERIUM ; SACCHAROPHAGUS-DEGRADANS ; GENE CLONING ; ENZYMATIC-HYDROLYSIS ; ALPHA-AGARASE ; ALBUS YKW-34 ; PURIFICATION ; EXPRESSION ; NEOAGAROBIOSE ; OLIGOSACCHARIDES
收录类别	SCI
语种	英语
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
项目资助者	National Natural Science Foundation of China(31471607 ; China Postdoctoral Science Foundation(2016M590661) ; 31271923)
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:000411043800017
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9584
专题	分子微生物工程研究组
作者单位	1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yunxiao,Ma, Xiaoqing,Zhang, Lujia,et al. Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(36):7982-7988.
APA	Liang, Yunxiao,Ma, Xiaoqing,Zhang, Lujia,Li, Fuli,Liu, Zhen,&Mao, Xiangzhao.(2017).Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(36),7982-7988.
MLA	Liang, Yunxiao,et al."Biochemical Characterization and Substrate Degradation Mode of a Novel Exotype beta-Agarase from Agarivorans gilvus WHO801".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.36(2017):7982-7988.