Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase

doi:10.1016/j.algal.2017.07.023

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	Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase
	Wei, Li 1; Wang, Qintao ; Xin, Yi ; Lu, Yandu ; Xu, Jian1
	2017-11-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	27 页码:366-375
摘要	Eukaryotic microalgae are promising cell factories for photosynthesis-driven CO2 sequestration and oil production, however one key challenge has been to genetically enhance their biomass productivity. Here we showed that the biomass productivity of a model industrial oleaginous microalga Nannochloropsis oceanica can be elevated by overexpression of a nuclear-encoded, cbbX-homologous, candidate RuBisCO activase (g1915 or 'nNoRca-like') that is transcriptionally induced under air-level CO2 (i.e., 0.04% v/v) as compared to 5% CO2 in wild-type cells. In the mutants, under air-level CO2, growth rate was enhanced by similar to 32%, biomass accumulation by similar to 46% and lipid productivity by similar to 41%, which were also accompanied by similar to 28% elevation in photosynthesis; furthermore, abundance of RuBisCO large subunit protein increased by similar to 45%, suggesting nNoRca-like overexpression enhanced microalgal photosynthesis by upregulating the level and activity of RuBisCO. Interestingly, under 5% CO2, no significant enhancement of RuBisCO protein expression was observed. Thus a genetic strategy that activates RuBisCO by augmenting its functional partners was proposed to increase biomass production of industrial microalgae. Our work expands the reservoir of potential photosynthetic gene targets for plant biotechnology and provides a basis for constructing advanced microalgal cell factories for carbon sequestration and biofuel production.
文章类型	Article
关键词	Industrial Oleaginous Microalgae Nannochloropsis Oceanica Rubisco Activase Cbbx Biomass Productivity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2017.07.023
关键词[WOS]	NANNOCHLOROPSIS-OCEANICA ; CYANIDIOSCHYZON-MEROLAE ; TRANSCRIPTION FACTOR ; NITROGEN-DEPLETION ; CARBON FIXATION ; CARBOXYLASE/OXYGENASE ; MECHANISMS ; EFFICIENCY ; DIVERSITY ; PLANTS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	Biological Carbon Sequestration Program from Chinese Academy of Sciences(ZDRW-ZS-2016-3 ; National Natural Science Foundation of China(31425002) ; National Basic Research Program from Chinese Ministry of Science and Technology(2012CB721101) ; KSZD-EW-Z-017)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000413256800039
引用统计	被引频次：87[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9565
专题	单细胞中心组群
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Single Cell Ctr,CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wei, Li,Wang, Qintao,Xin, Yi,et al. Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,27:366-375.
APA	Wei, Li,Wang, Qintao,Xin, Yi,Lu, Yandu,&Xu, Jian.(2017).Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,27,366-375.
MLA	Wei, Li,et al."Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 27(2017):366-375.