Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases

doi:10.1007/s00449-017-1791-1

QIBEBT-IR > 微藻生物技术研究组

	Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases
	Wang, Hui 1,2; Gao, Lili1,2 ; Shao, Huimin 1,3; Zhou, Wenjun1,2 ; Liu, Tianzhong1,2
	2017-09-01
发表期刊	BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷号	40 期号:9 页码:1327-1335
摘要	Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase. Using the genes involved in the lipid metabolism, the TAG biosynthesis pathways were generated. Moreover, results also demonstrated that, in addition to the observed overexpression of the fatty acid synthesis pathway, TAG production at stationary growth phase was bolstered by repression of the beta-oxidation pathway, up-regulation of genes that funnels acetyl-CoA to lipid biosynthesis, especially gene encoding for phospholipid:diacylglycerol acyltransferase (PDAT) which funnels DAG to TAG biosynthesis.
文章类型	Article
关键词	Tribonema Minus Confocal Image Lipid Synthesis Transcriptome Sequencing Differential Expression
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1007/s00449-017-1791-1
关键词[WOS]	CHLORELLA MINUTISSIMA ; BIODIESEL PRODUCTION ; BIOFUEL PRODUCTION ; MASS-SPECTROMETRY ; TRIACYLGLYCEROLS ; PERSPECTIVES ; ANNOTATION ; STRAIN ; GENES
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
项目资助者	Research Program of Application Foundation of Qingdao from Qingdao Science and Technology Bureau(16-5-1-68-jch) ; National Key R&D program from Ministry of Science and Technology of China(2016YFB0601001-02) ; Marine economic innovation and development regional demonstration project of Qingdao
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000407997600004
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9555
专题	微藻生物技术研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hui,Gao, Lili,Shao, Huimin,et al. Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2017,40(9):1327-1335.
APA	Wang, Hui,Gao, Lili,Shao, Huimin,Zhou, Wenjun,&Liu, Tianzhong.(2017).Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases.BIOPROCESS AND BIOSYSTEMS ENGINEERING,40(9),1327-1335.
MLA	Wang, Hui,et al."Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases".BIOPROCESS AND BIOSYSTEMS ENGINEERING 40.9(2017):1327-1335.