Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress

doi:10.1016/j.algal.2017.05.021

QIBEBT-IR > 代谢物组学研究组

	Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress
	Ma, Zengxin 1,4,5; Tian, Miaomiao 2; Tan, Yanzhen 1,6; Cui, Guzhen1,4 ; Feng, Yingang1,4 ; Cui, Qiu1,3,4 ; Song, Xiaojin1,4
	2017-07-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	25 页码:191-199
摘要	Aurantiochytrium is a commercial docosahexaenoic acid (DHA) producer, and its DHA content can be significantly increased under cold stress. Given this response to low temperature, we examined proteomics changes in Aurantiochytrium under cold stress. We detected approximately 700 protein spots using twodimensional gels, whereas using iTRAQ technology, we detected 4650 types of proteins and successfully identified> 53%. The results indicated that cold stress inhibits the cellular energy supply from glycolysis and the TCA cycle, to ensure a sufficient supply of NADPH and ribose for anabolism. In contrast, the pentose phosphate pathway was not affected. With respect to lipid synthesis, low temperature led to a significant downregulation and up-regulation of fatty acid synthase and polyunsaturated fatty acid synthase, respectively, and restricted the protein synthesis of diacylglycerol O-acyltransferase and phospholipid: diacylglycerol acyltransferase. These results show the preferential biosynthesis of polyunsaturated fatty acids and phospholipids by Aurantiochytrium, which collectively serve to increase cell survival rates in cold environments.
文章类型	Article
关键词	Aurantiochytrium Cold Stress Dha 2d Gels Itraq Proteome
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2017.05.021
关键词[WOS]	FATTY-ACIDS ; SCHIZOCHYTRIUM SP ; METABOLOMICS ANALYSIS ; PROTEOMIC ANALYSIS ; ANALYSIS REVEALS ; TEMPERATURE ; PROTEIN ; TRANSCRIPTOME ; ACCUMULATION ; GROWTH
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Key Research and Development Program(2016YFA0601400) ; National Natural Science Foundation of China(41306132 ; National High Technology Research and Development Program of China(2014AA021701) ; Qingdao Science and Technology Development Plan(14-2-4-69-jch) ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(QIBEBT-201503) ; 31500078)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000406305900023
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9549
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 4.Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Fourth Mil Med Univ, Xijing Hosp, Dept Cardiovasc Surg, Xian 710032, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Ma, Zengxin,Tian, Miaomiao,Tan, Yanzhen,et al. Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,25:191-199.
APA	Ma, Zengxin.,Tian, Miaomiao.,Tan, Yanzhen.,Cui, Guzhen.,Feng, Yingang.,...&Song, Xiaojin.(2017).Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,25,191-199.
MLA	Ma, Zengxin,et al."Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 25(2017):191-199.