Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

doi:10.1186/s12896-017-0351-8

QIBEBT-IR > 微生物代谢工程研究组

	Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship
	Wang, Qing 1,2; Bao, Luyao 1,2; Jia, Chenjun 2,3; Li, Mei 3; Li, Jian-Jun 1,4; Lu, Xuefeng 1
	2017-03-16
发表期刊	BMC BIOTECHNOLOGY
卷号	17
摘要	Background: Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k(cat) value below 1 min(-1), which would limit its application in biofuel production. To identify the activity related key residues of cADO is urgently required.
文章类型	Article
关键词	Aldehyde-deformylating Oxygenase Site-directed Mutagenesis Structure-activity Relationship Fatty Alk(A/e) Ne Synechococcus Elongatus Pcc7942 Synechocystis Sp Pcc6803
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1186/s12896-017-0351-8
关键词[WOS]	CYANOBACTERIAL ALDEHYDE ; FATTY ALDEHYDES ; DECARBONYLASE ; CONVERSION ; ALKANES ; INSIGHTS ; ENZYMES ; FORMATE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Basic Research Program of China (973)(2011CBA00907 ; National Science Foundation of China(31170765 ; CAS Cross and Cooperation Team for Scientific Innovation(Y31102110A) ; Shandong Natural Science Foundation(JQ201306) ; Shandong Taishan Scholarship ; 2011CBA00902) ; 31370799)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000396668000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9351
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qing,Bao, Luyao,Jia, Chenjun,et al. Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship[J]. BMC BIOTECHNOLOGY,2017,17.
APA	Wang, Qing,Bao, Luyao,Jia, Chenjun,Li, Mei,Li, Jian-Jun,&Lu, Xuefeng.(2017).Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship.BMC BIOTECHNOLOGY,17.
MLA	Wang, Qing,et al."Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship".BMC BIOTECHNOLOGY 17(2017).