| Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE) | |
Fang, Bo1,2; Xu, Huifang1 ; Liu, Yi1; Qi, Fengxia1; Zhang, Wei1; Chen, Hui1; Wang, Cong1; Wang, Yilin1,2; Yang, Wenxia1; Li, Shengying1
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| 2017-03-09 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| 卷号 | 7 |
| 摘要 | The cytochrome P450 enzyme OleT(JE) from Jeotgalicoccus sp. ATCC 8456 is capable of converting free long-chain fatty acids into alpha-alkenes via one-step oxidative decarboxylation in presence of H2O2 as cofactor or using redox partner systems. This enzyme has attracted much attention due to its intriguing but unclear catalytic mechanism and potential application in biofuel production. Here, we investigated the functionality of a select group of residues (Arg245, Cys365, His85, and Ile170) in the active site of OleTJE through extensive mutagenesis analysis. The key roles of these residues for catalytic activity and reaction type selectivity were identified. In addition, a range of heterologous redox partners were found to be able to efficiently support the decarboxylation activity of OleTJE. The best combination turned out to be SeFdx-6 (ferredoxin) from Synechococcus elongatus PCC 7942 and CgFdR-2 (ferredoxin reductase) from Corynebacterium glutamicum ATCC 13032, which gave the highest myristic acid conversion rate of 94.4%. Moreover, Michaelis-Menton kinetic parameters of OleTJE towards myristic acid were determined. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology |
| DOI | 10.1038/srep44258 |
| 关键词[WOS] | CYTOCHROME-P450 ENZYMES ; OXIDATIVE DECARBOXYLATION ; SUBSTRATE RECOGNITION ; BACILLUS-SUBTILIS ; CRYSTAL-STRUCTURE ; HYDROXYLATION ; PEROXYGENASE ; CHLOROPEROXIDASE ; PEROXIDASE ; ALKENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| 项目资助者 | National Science Foundation of China (NSFC)(31422002 ; Shandong Provincial Natural Science Foundation(JQ201407) ; Applied Basic Research Programs of Science and Technology of Qingdao(15-9-1-106-jch) ; 31270855 ; 21406250) |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000395976400001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9344 |
| 专题 | 酶工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Bo,Xu, Huifang,Liu, Yi,et al. Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Fang, Bo.,Xu, Huifang.,Liu, Yi.,Qi, Fengxia.,Zhang, Wei.,...&Li, Shengying.(2017).Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE).SCIENTIFIC REPORTS,7. |
| MLA | Fang, Bo,et al."Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)".SCIENTIFIC REPORTS 7(2017). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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