Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose

doi:10.1016/j.biortech.2016.11.110

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	Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose
	Zhang, Biao 1; Zhu, Yelin 1; Zhang, Jia 2; Wang, Dongmei1 ; Sun, Lianhong 1; Hong, Jiong 1
	2017
发表期刊	BIORESOURCE TECHNOLOGY
卷号	224 页码:553-562
摘要	Xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. In this study, the pyruvate decarboxylase gene (KmPDC1) and glycerol-3-phosphate dehydrogenase gene (KmGPD1) of Kluyveromyces marxianus YZJ051 were disrupted to prevent ethanol and glycerol accumulation. The deficient growth of PDC disruption was rescued by overexpressing mutant KmMTH1-Delta T. Then pentose phosphate pathway and xylitol dehydrogenase SsXYL2-ARS genes were overexpressed to obtain strain YZB053 which produced pyruvate with xylose other than glucose. It produced 24.62 g/L pyruvate from 80 g/L xylose with productivity of 0.51 g/L/ h at 42 degrees C. Then, xylose-specific transporter ScGAL2-N376F was overexpressed to obtain strain YZB058, which simultaneously consumed 40g/L glucose and 20 g/L xylose and produced 29.21 g/L pyruvate with productivity of 0.81 g/L/h at 42 degrees C. Therefore, a platform for pyruvate production from glucose and xylose at elevated temperature was developed. (C) 2016 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Kluyveromyces Marxianus Pyruvate Xylose Elevated Temperature Cofermentation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1016/j.biortech.2016.11.110
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; ETHANOL-PRODUCTION ; ESCHERICHIA-COLI ; SIMULTANEOUS SACCHARIFICATION ; XYLITOL PRODUCTION ; YEAST-STRAIN ; FERMENTATION ; PATHWAY ; THERMOTOLERANT ; OVEREXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者	China Postdoctoral Science Foundation(2016M590575) ; National Natural Science Foundation of China(31570082) ; Anhui Provincial Natural Science Foundation(1608085MC47)
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000395691900067
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9338
专题	单细胞中心组群
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Biao,Zhu, Yelin,Zhang, Jia,et al. Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose[J]. BIORESOURCE TECHNOLOGY,2017,224:553-562.
APA	Zhang, Biao,Zhu, Yelin,Zhang, Jia,Wang, Dongmei,Sun, Lianhong,&Hong, Jiong.(2017).Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose.BIORESOURCE TECHNOLOGY,224,553-562.
MLA	Zhang, Biao,et al."Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose".BIORESOURCE TECHNOLOGY 224(2017):553-562.