Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii

doi:10.1128/AEM.03230-16

QIBEBT-IR > 分子微生物工程研究组

	Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii
	Meng, Huimin 1,2; Wang, Zhangxun 1,3; Wang, Yulong 1; Zhu, Hong 1; Huang, Bo 1
	2017-04-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号	83 期号:7
摘要	RNA interference (RNAi) is a gene -silencing mechanism that plays an important role in gene regulation in a number of eukaryotic organisms. Two core components, Dicer and Argonaute, are central in the RNAi machinery. However, the physiological roles of Dicer and Argonaute in the entomopathogenic fungus Metarhizium robertsii have remained unclear. Here, the roles of genes encoding Dicer (M. robertsii dclI1 [Mrdcl1] and Mrdcl2) and Argonaute (Mrago1 and Mrogo2) proteins in M. robertsii were investigated. The results showed that the Dicer -like protein MrDCL2 and Argonaute protein MrAGO1 are the major components of the RNAi process occurring in M. robertsii. The Dicer and Argonaute genes were not involved in the regulation of growth and diverse abiotic stress response in M. robertsii under the tested conditions. Moreover, our results showed that the Dicer and Argonaute gene mutants demonstrated reduced abilities to produce conidia, compared to the wild type (WT) and the gene -rescued mutant. In particular, the conidial yields in the Delta dcl2 and Aogol mutants were reduced by 55.8% and 59.3%, respectively, compared with those from the control strains. Subsequently, for the \ATI and Delta dcl2 mutant strains, digital gene expression (DGE) profiling analysis of the stage of mycelium growth and conidiogenesis revealed that modest changes occur in development or metabolism processes, which may explain the reduction in conidiation in the Delta dcl2 mutant. In addition, we further applied high -throughput sequencing technology to identify small RNAs (sRNAs) that are differentially expressed in the WT and the Delta dcl2 mutant and found that 4 known microRNA-like small RNAs (milRNAs) and 8 novel milRNAs were Mrdcl2 dependent in M. robertsii.
文章类型	Article
关键词	Argonaute Dicer Metorhizium Robertsii Rna Interference
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1128/AEM.03230-16
关键词[WOS]	GENOME-WIDE IDENTIFICATION ; DOUBLE-STRANDED-RNA ; FILAMENTOUS FUNGI ; ASEXUAL SPORULATION ; SEQUENCE ALIGNMENT ; NEUROSPORA-CRASSA ; STRESS TOLERANCE ; MESSENGER-RNA ; ANISOPLIAE ; EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
项目资助者	National Natural Science Foundation of China(31272096 ; Anhui Provincial Natural Science Foundation(1308085QC61 ; Natural Science Foundation of the Higher Education Institutions of Anhui Province(KJ2015A081) ; 31471821 ; 1408085MKL37) ; 31572060)
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000397107300016
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9296
专题	分子微生物工程研究组
作者单位	1.Anhui Agr Univ, Anhui Prov Key Lab Microbial Pest Control, Hefei, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 3.Anhui Agr Univ, Sch Plant Protect, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Meng, Huimin,Wang, Zhangxun,Wang, Yulong,et al. Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(7).
APA	Meng, Huimin,Wang, Zhangxun,Wang, Yulong,Zhu, Hong,&Huang, Bo.(2017).Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(7).
MLA	Meng, Huimin,et al."Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.7(2017).