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Direct Analysis of Succinic Acid Fermentation Broth by H-1 Diffusion-Ordered NMR Spectroscopy and Quantitative H-1 NMR Technique
Wang, Limin2; Yang, Ying1; Yang, Haiyan1; Qiu, Ruchen2; Huang, Shaohua1
2017-04-01
发表期刊ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷号5期号:4页码:2824-2828
摘要The diffusion-ordered NMR spectroscopy (DOSY) protocol was first introduced to analyze directly the mixture samples of succinic acid fermentation broth. The NMR signals of the model mixtures and the practical mixture were facilely separated, and each component was fully assigned in the diffusion dimension of DOSY spectra. The composition of succinic acid fermentation broth could be rapidly determined according to the DOSY experiments. In contrast, this qualitative analysis could not be implemented through ion chromatography technique. On the basis of DOSY experimental results, the quantitative H-1 NMR (qHNMR) technique was further used to determinate successfully the components of one mixture sample of practical fermentation broth, which agreed well with the high-performance liquid chromatographic experimental result. The quantitative analysis results demonstrate that the DOSY technique and the qHNMR technique can determination of the product quality of succinic acid fermentation broth.
文章类型Article
关键词Succinic Acid Fermentation Broth Diffusion-ordered Nmr Spectroscopy Quantitative H-1 Nmr Technique Qualitative Analysis Quantitative Analysis
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1021/acssuschemeng.6b03184
关键词[WOS]RENEWABLE RESOURCES ; MATRIX
收录类别SCI
语种英语
WOS研究方向Chemistry ; Engineering
项目资助者National Natural Science Foundation of China(21105108) ; Chinese Academy of Sciences Key Technology Talent Program
WOS类目Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号WOS:000398429700005
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9294
专题公共实验室
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Chem Engn, 53 Zhengzhou Rd, Qingdao 266042, Peoples R China
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GB/T 7714
Wang, Limin,Yang, Ying,Yang, Haiyan,et al. Direct Analysis of Succinic Acid Fermentation Broth by H-1 Diffusion-Ordered NMR Spectroscopy and Quantitative H-1 NMR Technique[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2017,5(4):2824-2828.
APA Wang, Limin,Yang, Ying,Yang, Haiyan,Qiu, Ruchen,&Huang, Shaohua.(2017).Direct Analysis of Succinic Acid Fermentation Broth by H-1 Diffusion-Ordered NMR Spectroscopy and Quantitative H-1 NMR Technique.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,5(4),2824-2828.
MLA Wang, Limin,et al."Direct Analysis of Succinic Acid Fermentation Broth by H-1 Diffusion-Ordered NMR Spectroscopy and Quantitative H-1 NMR Technique".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 5.4(2017):2824-2828.
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