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DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation
Xu, Zongchang1,2; Wang, Meng1,2; Shi, Dachuan1; Zhou, Gongke3; Niu, Tiantian1; Hahn, Michael G.4,5; O'Neill, Malcolm A.4; Kong, Yingzhen1
2017-05-01
Source PublicationPLANT SCIENCE
Volume258Pages:156-169
AbstractOur previous study of the Arabidopsis mur3-3 mutant and mutant plants in which the mur3-3 phenotypes are suppressed (xxt2mur3-3, xxt5mur3-3, xxt1xxt2mur3-3 and 35Spro:XLT2:mur3-3) showed that hypocotyl cell elongation is decreased in plants that synthesize galactose-deficient xyloglucan. To obtain genome-wide insight into the transcriptome changes and regulatory networks that may be involved in this decreased elongation, we performed digital gene expression analyses of the etiolated hypocotyls of wild type (WT), mur3-3 and the four suppressor lines. Numerous differentially expressed genes (DEGs) were detected in comparisons between WT and mur3-3 (1423), xxt2mur3-3 and mur3-3 (675), xxt5mur3-3 and mur3-3 (1272), xxt1xxt2mur3-3 and mur3-3 (1197) and 35Spro:XLT2:mur3-3 vs mur3-3 (121). 550 overlapped DEGs were detected among WT vs mur3-3, xxt2mur3-3 vs mur3-3, xxt5mur3-3 vs mur3-3, and xxt1xxt2mur3-3 vs mur3-3 comparisons. These DEGs include 46 cell wall-related genes, 24 transcription factors, 6 hormone-related genes, 9 protein kinase genes and 9 aquaporin genes. The expression of all of the 550 overlapped genes is restored to near wild-type levels in the four mur3-3 suppressor lines. qRT-PCR of fifteen of these 550 genes showed that their expression levels are consistent with the digital gene expression data. Overexpression of some of these genes (XTH4, XTH30, PME3, EXPA11, MYB88, ROT3, AT5G37790, WAG2 and TIP2;3) that are down-regulated in mur3-3 partially rescued the short hypocotyl phenotype but not the aerial phenotype of mur3-3, indicating that different mechanisms exist between hypocotyl cell elongation and leaf cell elongation. (C) 2017 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordArabidopsis Mur3 Xyloglucan Cell Elongation Hypocotyl Dge-seq
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.plantsci.2017.01.005
WOS KeywordRNA-SEQ ; PLANT-GROWTH ; METHYL ESTERIFICATION ; FUCOSYL-TRANSFERASE ; ECTOPIC EXPRESSION ; WALL ; THALIANA ; GENE ; BIOSYNTHESIS ; AUXIN
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
Funding OrganizationNational Natural Science Foundation of China(31470291 ; National Key Technology RD Program(2015BAD15B03-05) ; Elite Youth Program of CAAS ; Taishan Scholar Program of Shandong ; Division of Chemical Sciences, Geosciences, and Bio-sciences, Office of Basic Energy Sciences of the U.S. Department of Energy(DE-FG02-12ER16324) ; 31670302)
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000399269700015
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9284
Collection资源植物与环境工程研究组
Affiliation1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China
2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
3.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
4.Univ Georgia, Complex Carbohydrate Res Ctr, 220 Riverbend Rd, Athens, GA 30602 USA
5.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA
Recommended Citation
GB/T 7714
Xu, Zongchang,Wang, Meng,Shi, Dachuan,et al. DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation[J]. PLANT SCIENCE,2017,258:156-169.
APA Xu, Zongchang.,Wang, Meng.,Shi, Dachuan.,Zhou, Gongke.,Niu, Tiantian.,...&Kong, Yingzhen.(2017).DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation.PLANT SCIENCE,258,156-169.
MLA Xu, Zongchang,et al."DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation".PLANT SCIENCE 258(2017):156-169.
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