DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation

doi:10.1016/j.plantsci.2017.01.005

QIBEBT-IR > 资源植物与环境工程研究组

	DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation
	Xu, Zongchang 1,2; Wang, Meng 1,2; Shi, Dachuan 1; Zhou, Gongke 3; Niu, Tiantian 1; Hahn, Michael G.4,5; O'Neill, Malcolm A.4; Kong, Yingzhen 1
	2017-05-01
发表期刊	PLANT SCIENCE
卷号	258 页码:156-169
摘要	Our previous study of the Arabidopsis mur3-3 mutant and mutant plants in which the mur3-3 phenotypes are suppressed (xxt2mur3-3, xxt5mur3-3, xxt1xxt2mur3-3 and 35Spro:XLT2:mur3-3) showed that hypocotyl cell elongation is decreased in plants that synthesize galactose-deficient xyloglucan. To obtain genome-wide insight into the transcriptome changes and regulatory networks that may be involved in this decreased elongation, we performed digital gene expression analyses of the etiolated hypocotyls of wild type (WT), mur3-3 and the four suppressor lines. Numerous differentially expressed genes (DEGs) were detected in comparisons between WT and mur3-3 (1423), xxt2mur3-3 and mur3-3 (675), xxt5mur3-3 and mur3-3 (1272), xxt1xxt2mur3-3 and mur3-3 (1197) and 35Spro:XLT2:mur3-3 vs mur3-3 (121). 550 overlapped DEGs were detected among WT vs mur3-3, xxt2mur3-3 vs mur3-3, xxt5mur3-3 vs mur3-3, and xxt1xxt2mur3-3 vs mur3-3 comparisons. These DEGs include 46 cell wall-related genes, 24 transcription factors, 6 hormone-related genes, 9 protein kinase genes and 9 aquaporin genes. The expression of all of the 550 overlapped genes is restored to near wild-type levels in the four mur3-3 suppressor lines. qRT-PCR of fifteen of these 550 genes showed that their expression levels are consistent with the digital gene expression data. Overexpression of some of these genes (XTH4, XTH30, PME3, EXPA11, MYB88, ROT3, AT5G37790, WAG2 and TIP2;3) that are down-regulated in mur3-3 partially rescued the short hypocotyl phenotype but not the aerial phenotype of mur3-3, indicating that different mechanisms exist between hypocotyl cell elongation and leaf cell elongation. (C) 2017 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Arabidopsis Mur3 Xyloglucan Cell Elongation Hypocotyl Dge-seq
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.plantsci.2017.01.005
关键词[WOS]	RNA-SEQ ; PLANT-GROWTH ; METHYL ESTERIFICATION ; FUCOSYL-TRANSFERASE ; ECTOPIC EXPRESSION ; WALL ; THALIANA ; GENE ; BIOSYNTHESIS ; AUXIN
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
项目资助者	National Natural Science Foundation of China(31470291 ; National Key Technology RD Program(2015BAD15B03-05) ; Elite Youth Program of CAAS ; Taishan Scholar Program of Shandong ; Division of Chemical Sciences, Geosciences, and Bio-sciences, Office of Basic Energy Sciences of the U.S. Department of Energy(DE-FG02-12ER16324) ; 31670302)
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000399269700015
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9284
专题	资源植物与环境工程研究组
作者单位	1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China 2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 4.Univ Georgia, Complex Carbohydrate Res Ctr, 220 Riverbend Rd, Athens, GA 30602 USA 5.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA
推荐引用方式 GB/T 7714	Xu, Zongchang,Wang, Meng,Shi, Dachuan,et al. DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation[J]. PLANT SCIENCE,2017,258:156-169.
APA	Xu, Zongchang.,Wang, Meng.,Shi, Dachuan.,Zhou, Gongke.,Niu, Tiantian.,...&Kong, Yingzhen.(2017).DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation.PLANT SCIENCE,258,156-169.
MLA	Xu, Zongchang,et al."DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation".PLANT SCIENCE 258(2017):156-169.