Pickering Emulsion as an Efficient Platform for Enzymatic Reactions without Stirring

doi:10.1021/acssuschemeng.6601776

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	Pickering Emulsion as an Efficient Platform for Enzymatic Reactions without Stirring
	Wei, Lijuan 1; Zhang, Ming 1; Zhang, Xiaoming 1; Xin, Hongchuan 2; Yang, Hengquan 1
	2016-12-01
发表期刊	ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷号	4 期号:12 页码:6838-6843
摘要	To address the current limitations of enzymatic reactions, we develop a novel strategy to conduct stirring-free biphasic enzymatic reactions. This strategy involves translation of a conventional biphasic enzymatic reaction to a water-in-oil (W/O) Pickering emulsion system by adding a small amount of solid particle emulsifier. In such a system, enzymes, for example, a Candida Antarctica lipase B (CALB), are compartmentalized within millions of micron-sized water droplets, while organic substrates are dissolved in the oil phase (outside the droplets). It was demonstrated that CALB-catalyzed hydrolysis kinetic resolution of racemic esters in the stirring-free Pickering emulsion system gave favorable reaction efficiency and enantioselectivity as compared to those for the conventional biphasic system under stirring conditions, which was due to the large reaction interfacial area and the short molecule distances created by the Pickering emulsion droplets. The specific activity was found to depend on the water droplet size, highlighting the importance of the presence of droplets in the reaction system. Moreover, the convenient and effective recycling of CALB could be achieved through simple demulsification by centrifugation. After 27 reaction cycles, the ee values of ester and alcohol were still as high as 87.5% and 99%, respectively, which significantly exceed those of the conventional biphasic reaction. The high recyclability may be attributed to avoiding stirring that often causes damage to the three-dimensional structure of enzymes. This study compellingly demonstrates that a Pickering emulsion is an innovative platform to efficiently process enzymatic reactions without need for stirring and immobilization.
文章类型	Article
关键词	Pickering Emulsion Enzymatic Reactions Hydrolysis Kinetic Resolution
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1021/acssuschemeng.6601776
关键词[WOS]	DYNAMIC KINETIC RESOLUTION ; INTERFACIAL BIOCATALYSTS ; BIPHASIC BIOCATALYSIS ; SECONDARY ALCOHOLS ; CANDIDA-ANTARCTICA ; CASCADE REACTIONS ; PHASE-TRANSFER ; LIPASE B ; IMMOBILIZATION ; PARTICLES
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Engineering
项目资助者	Natural Science Foundation of China(21173137 ; Program for New Century Excellent Talents in University(NECT-12-1030) ; foundation for Young Sanjin Scholar ; State Key Laboratory of Coal Conversions (INSTITUTE of COAL CHEMISTRY)(J15-16-609) ; 21573136)
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000389497900066
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9207
专题	科技处
作者单位	1.Shanxi Univ, Sch Chem & Chem Engn, Taiyuan 030006, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Wei, Lijuan,Zhang, Ming,Zhang, Xiaoming,et al. Pickering Emulsion as an Efficient Platform for Enzymatic Reactions without Stirring[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2016,4(12):6838-6843.
APA	Wei, Lijuan,Zhang, Ming,Zhang, Xiaoming,Xin, Hongchuan,&Yang, Hengquan.(2016).Pickering Emulsion as an Efficient Platform for Enzymatic Reactions without Stirring.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,4(12),6838-6843.
MLA	Wei, Lijuan,et al."Pickering Emulsion as an Efficient Platform for Enzymatic Reactions without Stirring".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 4.12(2016):6838-6843.