Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116

doi:10.1016/j.algal.2016.08.005

QIBEBT-IR > 代谢物组学研究组

	Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116
	Cui, Gu-Zhen 1; Ma, Zengxin 1,2; Liu, Ya-Jun 1; Feng, Yingang 1; Sun, Zhijie 1; Cheng, Yurong 1,2; Song, Xiaojin 1; Cui, Qiu 1
	2016-11-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	19 页码:138-145
摘要	Aurantiochytrium sp. (Thraustochytriaceae, Stramenopiles) is a highly promising polyunsaturated fatty acid (PUFA) resource due to its high docosahexaenoic acid (DHA) production. The reducing agent NADPH is essential for the production of PUFA and other fatty acids, and glucose-6-phosphate dehydrogenase (G6PDH) is one of the most important enzymes for NADPH regeneration in Aurantiochytrium. To investigate whether PUFA production could be enhanced by stimulating G6PDH, a recombinant strain, termed SD116::G6PDH, was constructed by overexpressing G6PDH in Aurantiochytriumsp. SD116. Although decreased cell growth and fatty acid production were observed, G6PDH overexpression changed the fatty acid profile and enhanced the proportion of PUFA in lipids by 10.6%. These results confirmed the positive effect of G6PDH activity on PUFA production and suggested that the manipulation of NADPH production is a promising strategy for increasing PUFA production in Aurantiochytrium. (C) 2016 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Aurantiochytrium Polyunsaturated Fatty Acid Glucose-6-phosphate Dehydrogenase Nadph
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2016.08.005
关键词[WOS]	GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; DOCOSAHEXAENOIC ACID ; MALIC ENZYME ; MICROALGA SCHIZOCHYTRIUM ; TAXONOMIC REARRANGEMENT ; TRANSGENE EXPRESSION ; MUCOR-CIRCINELLOIDES ; LIPID-METABOLISM ; SYSTEM ; DHA
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China(41306132 ; National High Technology Research and Development Program of China(2014AA021701) ; Qingdao Science and Technology Development Plan(14-2-4-69-jch) ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(QIBEBT-201503) ; 31500078)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000390001400017
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9205
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet,Key Lab Biofue, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Gu-Zhen,Ma, Zengxin,Liu, Ya-Jun,et al. Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,19:138-145.
APA	Cui, Gu-Zhen.,Ma, Zengxin.,Liu, Ya-Jun.,Feng, Yingang.,Sun, Zhijie.,...&Cui, Qiu.(2016).Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,19,138-145.
MLA	Cui, Gu-Zhen,et al."Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 19(2016):138-145.