Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor

doi:10.1016/j.bios.2016.07.050

QIBEBT-IR > 环境资源化与水回用研究组

	Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor
	Liu, Aihua 1,2,3; Lang, Qiaolin1,2,3,4 ; Liang, Bo4 ; Shi, Jianguo 3
	2017-01-15
发表期刊	BIOSENSORS & BIOELECTRONICS
卷号	87 页码:25-30
摘要	Glucoamylase-displayed bacteria (GA-bacteria) and glucose dehydrogenase-displayed bacteria (GDH-bacteria) were co-immobilized on multi-walled carbon nanotubes (MWNTs) modified glassy carbon electrode (GCE) to construct GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor. The biosensor was developed by optimizing the loading amount and the ratio of GA-bacteria to GDH-bacteria. The as prepared biosensor exhibited a wide dynamic range of 0.2-10 mM and a low detection limit of 0.1 mM maltose (S/N=3). The biosensor also had a linear response to glucose in the range of 0.1-2.0 mM and a low detection limit of 0.04 mM glucose (S/N=3). Interestingly, at the same concentration, glucose was 3.75-fold sensitive than that of maltose at the proposed biosensor. No interferences were observed for other possible mono- and disaccharides. The biosensor also demonstrated good long-term storage stability and repeatability. Further, using both GDH-bacteria/MWNTs/GCE biosensor and GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor, glucose and maltose in real samples can be detected. Therefore, the proposed biosensor is capable of monitoring the food manufacturing and fermentation process. (C) 2016 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Glucoamylase Displayed Bacteria Glucose Dehydrogenase Displayed Bacteria Maltose Biosensor Glucose And Maltose Detection Saccharide Analysis
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.bios.2016.07.050
关键词[WOS]	WHOLE-CELL BIOCATALYST ; XYLOSE DEHYDROGENASE ; BIOFUEL CELL ; CARBON NANOTUBES ; CONDUCTOMETRIC BIOSENSOR ; SURFACE DISPLAY ; FLOW-INJECTION ; ELECTRODE ; CONSTRUCTION ; OXIDASE
收录类别	SCI
语种	英语
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
项目资助者	National Natural Science Foundation of China(21475144 ; 21275152 ; 31500799)
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000390499600005
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9194
专题	环境资源化与水回用研究组
作者单位	1.Qingdao Univ, Inst Biosensing, 308 Ningxia Rd, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Chem & Chem Engn, 308 Ningxia Rd, Qingdao 266071, Peoples R China 3.Key Lab Biosensors Shangdong Prov, Jinan, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Aihua,Lang, Qiaolin,Liang, Bo,et al. Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor[J]. BIOSENSORS & BIOELECTRONICS,2017,87:25-30.
APA	Liu, Aihua,Lang, Qiaolin,Liang, Bo,&Shi, Jianguo.(2017).Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor.BIOSENSORS & BIOELECTRONICS,87,25-30.
MLA	Liu, Aihua,et al."Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor".BIOSENSORS & BIOELECTRONICS 87(2017):25-30.