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Title:
Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment
Author: Zhang, Ning1,2,3; An, Liaoyuan1,2; Li, Jingwen1,2,3; Liu, Zhijun4; Yao, Lishan1,2
Source: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Issued Date: 2017-01-18
Volume: 139, Issue:2, Pages:647-654
DOI: 10.1021/jacs.6b11058
DOC Type: Article
English Abstract: The intramolecular electric field (e-field) generated by protein GB3 side-chain charges K/E10, K/E19, and D/K40 was measured in the absence or presence of macromolecular crowding. The e-field responds differently to different crowding agents-dextran, Ficoll, BSA, and E. coli cell lysate. Dextran and Ficoll have no effect on the e-field. The lysate generally weakens the e-field but the amplitude of weakening varies greatly. For example, the e-field by K19 is reduced by 67% in the presence of 90 g/L lysate, corresponding to a charge change from 0.9 to 0.3 e for K19, whereas the e-fields by D/K40 are weakened only by, similar to 7% under the same lysate concentration. The extent of the e-field weakening by BSA is in between that by Ficoll (dextran) and lysate. Further investigations, suggest that the e-field weakening mechanism by lysate is similar to that by NaCl. That is, the e-field generated by a protein surface charge affects the distribution of lysate which creates a reaction field and weakens the protein e-field. Our study indicates that the protein electrostatic property can be changed significantly due to quinary interaction with the cell environment.
WOS Headings: Science & Technology ; Physical Sciences
WOS Subject: Chemistry, Multidisciplinary
WOS Subject Extended: Chemistry
WOS Keyword Plus: RESIDUAL DIPOLAR COUPLINGS ; NUCLEAR-MAGNETIC-RESONANCE ; NMR-SPECTROSCOPY ; LIVING CELLS ; CONFORMATIONAL EXCHANGE ; ESCHERICHIA-COLI ; LIQUID-CRYSTAL ; STABILITY ; DYNAMICS ; ANISOTROPY
Indexed Type: SCI
Funder: National Natural Science Foundation of China(31270785 ; 31270905 ; 31600690)
Language: 英语
WOS ID: WOS:000392459300021
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/9167
Appears in Collections:仿真与模拟团队_期刊论文

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description.institution: 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266061, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai 201210, Peoples R China

Recommended Citation:
Zhang, Ning,An, Liaoyuan,Li, Jingwen,et al. Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(2):647-654.
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